Expression of HPV-DNA and P16 protein in laryngeal cancer, benign laryngeal lesions, and vocal cord polyps
10.12025/j.issn.1008-6358.2017.20170552
- VernacularTitle:喉癌、喉良性病变及声带息肉组织HPV-DNA及P16蛋白的表达差异
- Author:
Qiang LI
1
;
Xue-Mei QIN
;
Xuan-Guang YE
;
Li-Xin GU
;
Xiao-Ping ZHU
Author Information
1. 复旦大学附属金山医院耳鼻咽喉-头颈外科
- Keywords:
laryngeal cancer;
benign laryngeal lesions;
vocal cord polyp;
human papillomavirus (HPV);
real-time fluorescence PCR
- From:
Chinese Journal of Clinical Medicine
2017;24(5):744-747
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression of HPV-DNA and P16 protein in laryngeal cancer,laryngeal benign lesion tissue,and vocal cord polyp tissue,and to explore the correlation between HPV infection and laryngeal cancer.Methods:The clinical data of 256 patients who had been treated in Department of Otorhinolaryngology & Head and Neck Surgery of Jinshan Hospital,Fudan University from May 2011 to July 2015 were collected.There were 34 cases of laryngeal squamous cell carcinoma,15 cases of benign laryngeal lesions (non-vocal cord polyps),207 cases of vocal cord polyps,184 males and 72 females,with a median age of 56 years.The levels of HPV-DNA in laryngeal squamous cell carcinoma tissues and benign laryngeal lesions were detected by real-time fluorescence PCR.Immunohistochemical staining was used to detect the expression of P16 protein in laryngeal cancer tissues,laryngeal benign lesions and vocal cord polyps.Results:Real time fluorescence PCR detection showed that the HPV DNA positive rate in 34 cases of laryngeal squamous cell carcinoma was 8.8％ (3/34),and it was all negative (0/15) in benign laryngeal lesions.There was no significant difference in the HPV-DNA positive rate between the two groups.Immunohistochemical staining showed that the positive expression rate of P16 protein in laryngeal squamous cell carcinoma,benign laryngeal lesions and vocal cord polyp tissues was 32.4％ (11/34),46.7％ (7/15),and 47.8％ (99/207).The positive rate of P16 protein in vocal cord polyp tissues was higher than that of laryngeal cancer tissues,and the difference was statistically significant (P=0.046).Conclusions:The positive rate of HPV-DNA in laryngeal cancer tissues is slightly higher than that in benign laryngeal lesions.The expression of P16 protein in laryngeal cancer tissues is slightly lower than that in benign laryngeal lesions,and is significantly lower than that in vocal cord polyps.
