Lipid metabolic characteristics of functionally exhausted CD8+T cells in tumor microenvironment of hepatocellular carcinoma
10.12025/j.issn.1008-6358.2017.20170621
- VernacularTitle:肝细胞肝癌肿瘤微环境中功能耗竭CD8+T细胞脂代谢特征的观察
- Author:
Qi SHEN
1
;
Le-Ying ZHUO
;
Fang-Ming LIU
;
Miao-Miao ZHANG
;
Han WANG
;
Wei-Ren LIU
;
Duo-Jiao WU
Author Information
1. 复旦大学附属中山医院实验研究中心
- Keywords:
hepatocellular carcinoma;
CD8+T cells;
metabolism;
functional exhaustion;
microenvironment
- From:
Chinese Journal of Clinical Medicine
2017;24(4):514-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the lipid metabolic characteristics of functionally exhausted CD8+T cells in tumor microenvironment of hepatocellular carcinoma(HCC).Methods:By using flow cytometry analysis and real-time PCR to detect the functions and lipid metabolism-related proteins and genes expression of CD8+T cells in HCC and peri-tumor tissues,then the relationship between function and lipid metabolism was analyzed.Objective:The infiltrated CD8+T cells in HCC have the following characteristics: functional exhaustion(decreased interferon-γ secretion and up regulated programmed death molecule-1 expression);down regulated expression of multiple fatty acid synthases,and the decreased content of intracellular fatty acids;impaired mitochondrial structure and function(P<0.05).Conclusions:Fatty acid metabolism abnormalities are the characteristics of exhausted CD8+T cell infiltrated in HCC.
