Experimental study on the effects of mechanical stretch on the skin graft
10.3760/cma.j.cn114453-20200304-00105-1
- VernacularTitle:A型肉毒毒素不同干预方式对移植皮片术后挛缩的影响
- Author:
Jinfeng ZHOU
1
;
Chao HAO
;
Wengbo YANG
Author Information
1. 南京医科大学附属南京医院（南京市第一医院）烧伤整形科 210006
- Keywords:
Botulinum toxins, type A;
Rat;
Skin graft;
Contracture
- From:
Chinese Journal of Plastic Surgery
2021;37(11):1265-1270
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the anticontracture effect of injecting botulinum toxin Type A(BTX-A) at different sites after skin grafting.Methods:24 SD rats of SPF grade were divided into three groups. Two full-thickness skin grafts of the same size were performed on each rat’s back. One was randomly selected as the treatment side and the other as the control side. BTX-A was used for edge injection, substrate injection and intradermal injection on the treatment side of each group. No intervention was done on the control side.The area of grafts was measured at the end of the 2nd, 4th, 6th, 8th, 10th and 12th weeks after surgery. The differences between treatment groups were analyzed by repeated measures of variance, P<0.05 was considered statistically significant. Results:The area of skin grafts on the treatment side and the control side in the edge injection group was significantly different, except that there was no statistical difference at the end of the 2nd weeks( at 4, 6, 8, 10, and 12 weeks P<0.01). In the 12th week, the average area of the experimental side in this group was (4.106±0.320) cm 2, while the average area of the control side was (2.885±0.264) cm 2. There was no significant difference between the two sides in the intradermal injection group at each postoperative interval( P> 0.05). At the end of the 12th week, the average area of the experimental side in the intradermal injection group was (3.443±0.678) cm 2, while the average area of the control side was (3.503±0.577) cm 2. The results were different in the substrate injection group.The area of skin grafts on the treatment side was larger than that on the control side, but there was no statistical difference at the end of the 2nd, 4th and 6th weeks after the operation( P>0.05). At the end of the 8th, 10th, and 12th weeks, the differences were statistically significant( P<0.05, <0.01, <0.05). In the 12th week, the average area of the experimental side in this group was(3.660±0.434)cm 2, while the average area of the control side was (2.914±0.528) cm 2. Conclusions:The preliminary results showed that BTX-A injection at the edge and base of the wound could inhibit the contracture after skin grafting. The injection at the edge of the wound was more effective. Use of BTX-A for anticontracture after skin graft is worthy further study.
