The intervention effect of bone marrow-derived mesenchymal stem cells on inflammatory factors in lung tissue and plasma of phosgene inhalation lung injury rats
10.12025/j.issn.1008-6358.2017.20170124
- VernacularTitle:骨髓间充质干细胞移植对光气吸入性肺损伤大鼠肺组织及血浆炎症因子的干预作用
- Author:
Si-Qing MIN
1
;
Dai-Kun HE
;
Zhi-Yue ZHONG
;
Jun-Feng CHEN
;
Jie SHEN
Author Information
1. 复旦大学附属金山医院重症监护室
- Keywords:
phosgene;
acute lung injury;
cytokines;
bone marrow-derived mesenchymal stem cells
- From:
Chinese Journal of Clinical Medicine
2017;24(3):400-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the intervention effect of bone marrow-derived mesenchymal stem cells (BMD-MSCs) on inflammatory factors in lung tissue and plasma of phosgene inhalation lung injury rats.Methods:96 male SD rats were randomly divided into air control group (group A), BMD-MSCs intervention control group (group AM), phosgene exposure group (group PH) and BMD-MSCs intervention group after phosgene exposure (group PM), with 8 rats in each group.Group PH was exposed to dynamic constant phosgene at 8.33 mg/L for 5 min.Group PM was injected with 106 BMD-MSCs via the tail vein immediately after phosgene exposure.The rats in the 4 groups were sacrificed at 6, 24 and 48 h after the models were completed.The lung tissues were taken for pathological examination, lung wet / dry ratio and blood gas analysis.The plasma was taken for the Bio-Plex cytokine test.Results:The level of TNF-α was higher in group PH than group A, and the difference was statistically significant (P=0.02).The level of TNF-α was lower in the group PM than group PH (P<0.05).The plasma IL-4 concentration in group PM and group PH decreased by 6h and 24 h, which was more significant in group PM (P<0.05).It increased significantly by 48h, which was more significant in group PM (P<0.05).The level of IL-10 was higher in group PM than group PH, and the difference was statistically significant (P<0.05).The level of IL-10 was higher in group PM than group PH, and the difference was statistically significant (P<0.05).Conclusions:Anti-inflammatory cytokines IL-10 and IL-4 were increased and pro-inflammatory cytokines TNF-α was decreased by the tail vein injection of BMD-MSCs, so BMD-MSCs has a protective effect in rats with acute lung injury induced by phosgene.
