The angiogenic effect and potential mechanism of sodium tanshinone ⅡA sulfonate in ischemic zebrafish model
10.12025/j.issn.1008-6358.2017.20160674
- VernacularTitle:丹参酮ⅡA磺酸钠在缺血性斑马鱼模型中的促血管新生作用
- Author:
Fei CHEN
1
;
Qi QIAO
;
Zhang-Sheng WANG
;
Peng XU
;
Gang LU
Author Information
1. 复旦大学附属上海市第五人民医院心血管内科
- Keywords:
sodium tanshinonⅡA sulfonate;
angiogenesis;
zebrafish;
vasculaendothelial growth factor
- From:
Chinese Journal of Clinical Medicine
2017;24(2):166-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the angiogenic effect and potential mechanism of sodium tanshinone ⅡA sulfonate (Tan ⅡA) in ischemic zebrafish model.Methods:Vascular endothelial growth factor receptor kinase inhibitor (VRKI) was used to induce vascular injury in zebrafish model.Then the ischemic zebrafish model was treated with 50, 100, 200 μmol/L Tan ⅡA, respectively.The effects of Tan ⅡA on ischemic zebrafish subintestinal veins (SIVs) and intersegmental vessels (ISVs) were observed.Meanwhile, the expression changes of three vascular endothelial growth factor (VEGF) signaling pathway-related genes (flt-1, flk-1A, flk-1B) were detected by real-time PCR.Results:In VRKI-induced vascular injury zebrafish model, Tan ⅡA gradually restored ISVs and SIVs in a concentration-dependent manner, reversed the down-regulated expressions of three key VEGF signaling pathway-related genes (flt-1, flk-1A, flk-1B), and promoted angiogenesis.Conclusions:In the ischemic zebrafish model, Tan ⅡA plays a role in angiogenesis and vascular protection by regulating VEGF signaling pathway.
