Hypoxia promotes the invasion of cervical cancer cells in vitro by stimulating secretion of TSLP
10.12025/j.issn.1008-6358.2017.20170114
- VernacularTitle:缺氧通过刺激胸腺基质淋巴细胞生成素分泌促进宫颈癌细胞体外侵袭
- Author:
Kai-Kai CHANG
1
;
Hui-Li YANG
;
Wen-Jie ZHOU
;
Ming-Qing LI
;
Feng XIE
Author Information
1. 复旦大学附属妇产科医院研究所生殖免疫研究室
- Keywords:
thymic stromal lymphopoietin;
hypoxia;
cervical cancer;
invasion
- From:
Chinese Journal of Clinical Medicine
2017;24(2):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of hypoxia and thymic stromal lymphopoietin (TSLP) on the invasion of cervical cancer cells in vitro.Methods:Enzyme linked immunosorbent assay (ELISA) was performed to analyze the effects of normoxia and hypoxia on the secretion level of TSLP, and to analyze the effects of recombinant human TSLP (rhTSLP) protein on the expression levels of matrix metalloprotein-2 (MMP-2), MMP-9, and IL-8 in cervical cancer cell lines HeLa and CaSki.Matrigel invasion assay was used to investigate the effects of rhTSLP, hypoxia, and anti-human TSLP (aTSLP) neutralizing antibody (aTSLP, 5 μg/mL) on the invasiveness of HeLa and CaSki cells in vitro.Results:Under hypoxia exposure, the levels of TSLP secretion in HeLa and CaSki cells were significantly increased (P<0.01).RhTSLP (100 ng/mL) stimulation in vitro significantly enhanced the invasiveness of HeLa and CaSki cells (P<0.001), and promoted the productions of MMP-2, MMP-9, and IL-8 (P<0.01).The invasiveness of HeLa and CaSki cells can be promoted significantly under hypoxia exposure (P<0.001).In contrast, aTSLP (5 μg/mL) inhibited the invasiveness of HeLa and CaSki cells (P<0.05), and partly inhibited the stimulatory effect of hypoxia on invasiveness of HeLa and CaSki cells (P<0.05).Conclusions:Hypoxia facilitates the invasion of cervical cancer cells in vitro by up-regulating the secretion of TSLP.
