The role of microRNAs in activation of epithelial-mesenchymal transition in non-small-cell lung cancer by forkhead box M1
10.12025/j.issn.1008-6358.2016.20160990
- VernacularTitle:微小RNA在叉头转录因子M1激活非小细胞肺癌上皮向间质转化中的作用
- Author:
Yan WU
1
;
Li-Fei ZOU
;
Fu-Xin HUI
;
Jia-Kun WANG
Author Information
1. 南京医科大学附属无锡人民医院呼吸科
- Keywords:
forkhead box M1;
epithelial-mesenchymal transition;
NSCLC;
microRNA
- From:
Chinese Journal of Clinical Medicine
2016;23(6):739-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of microRNAs in Fox M1‐induced non‐small cell lung cancer (NSCLC) epithelial‐mesenchymal transition (EMT) .Methods :Over expression Fox M1 plasmid ,Fox M1‐shRNA ,miR‐539 ,miR‐485‐5p mimics and inhibitor were transfected into NSCLC cells .The expression of Fox M1 protein and mRNA ,miR‐539 and miR‐485‐5p were detected by Western blotting and real‐time PCR .The CCK‐8 and cell migration was used to observe the effect of Fox M1 ,miR‐539 and miR‐485‐5p on the proliferation and invasion of NSCLC .Luciferase reporter assay was used to explore the relationship between miRNA ( miR‐539 and miR‐485‐5p) and EM T regulatory protein (ZEB1 and Snail1 ) .Results:Real‐time RT‐PCR and Western blotting showed Fox M1 over expression inhibited the expression of miR‐539 ,miR‐485‐5p ,miR‐539 and miR‐485‐5p in NSCLC cells were increased after transfected Fox M 1‐shRNA . MiR‐539 and miR‐485‐5p suppressed enhancement of proliferation and invasion of NSCLC cells by Fox M 1 .miR‐539 targeted and inhibited the translation of ZEB1 , miR‐485‐5p targeted and inhibited the translation of Snail1 in NSCLC cells .Conclusions : The mechanism of Fox M1 up‐regulation of EM T protein is to decrease the expression of miR‐539 and miR‐485‐5p in NSCLC cells , thus to affect the proliferation and invasion of NSCLC cells ,and to inhibit distant metastasis .
