Application of touch imprint cytology plus rapid immunohistochemistry staining in the intraoperative assessments of breast cancer sentinel lymph nodes (SLNs)
10.12025/j.issn.1008-6358.2016.20160630
- VernacularTitle:印片细胞学联合快速免疫组化检测在乳腺癌前哨淋巴结活检术中诊断的应用
- Author:
Shu-Ling ZHU
1
;
Tong-Tong WU
;
Qin FANG
;
Qing-Xu YANG
Author Information
1. 惠州市中心人民医院病理科
- Keywords:
breast cancer;
sentinel lymph node;
touch imprint cytology;
rapid immunohistochemistry staining
- From:
Chinese Journal of Clinical Medicine
2016;23(5):633-635
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the clinical values of touch imprint cytology (TIC)plus rapid immunohistochemistry staining (RIHC)in the intraoperative assessments of breast cancer sentinel lymph nodes (SLNs).Methods:A total of 281 SLNs from 89 patients of breast cancer were examined in this study.All those SLNs were evaluated with intraoperative touch imprint cytology and rapid immunohistochemistry staining.The RIHC indicator was pan-cytokeratin 1 9 (pan-CK 1 9 ).The two intraoperative methods are compared the results of SLNs metastases by postoperative routine pathological examination.Results:The detection rate of SLNs with TIC was 25.8% (23/89 );the detection rate with touch imprint cytology plus rapid immunohistochemistry staining technique was 36.0%(32/89),which was much higher than touch imprint cytology(P<0.05).The specificity was both 100.0%.The sensitivity of TIC and TIC plus RIHC for detecting positive lymph node was 69.7% (23/33)and 97.0% (32/33),respectively.The sensitivity of TIC plus RIHC was significantly higher than that of TIC (P<0.001).The false negative rate of SLNs with TIC was 30.3%(10/33);the false negative rate with TIC plus RIHC was 3.0%(1/33),which was lower than TIC (P<0.001).Conclusions:Touch imprint cytology plus rapid immunohistochemistry staining can provide a reliable and precise result for the intraoperative assessment of breast cancer SLNs.
