Protective effects of melatonin on lung inj ury induced by lipopolysaccharide in rats with sepsis
- VernacularTitle:褪黑素对脂多糖诱导的脓毒症大鼠肺损伤的保护作用
- Author:
Jie-Qiong SONG
1
;
Wei WU
;
Song CHEN
;
Du-Ming ZHU
;
Ming ZHONG
Author Information
1. 复旦大学附属中山医院重症医学科
- Keywords:
melatonin;
lipopolysaccharide;
sepsis;
tumor necrosis factor-α;
interleukin
- From:
Chinese Journal of Clinical Medicine
2016;23(5):550-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effects of melatonin (MT)on lung injury induced by lipopolysaccharide (LPS)in rats with sepsis.Methods:Seventy-two rats were randomly divided into four groups with 18 rats in each group:control group (0.9%NS,i.v.),LPS group (LPS 1 mg/kg,i.v.),LPS+MT1 group (MT 0.1 mg/kg+LPS 1 mg/kg,i.v.)and LPS+MT2 group (MT 1 mg/kg+LPS 1 mg/kg,i.v.).Carotid arterial blood of all rats was collected at 6 h after administration and serum levels of tumor necrosis factor-α(TNF-α),interleukin-6 (IL-6)and interleukin-10 (IL-10) were detected by enzyme linked immunosorbent assay (ELISA ).The lung homogenate contents of superoxide dismutase (SOD),malondialdehyde(MDA)and myeloperoxidase (MPO)were also examined.Results:Compared with control group, the levels of serum TNF-α,IL-6 and IL-10 were significant higher in LPS group (P<0.01).With the MT intervention,the levels of TNF-αand IL-6 were significantly decreased but the IL-10 level was remarkably increased (P<0.05).This effect was more remarkable with the increase of the dose of MT.The level of SOD in LPS group was remarkably lower than in control group,but the levels of MDA and MPO were significantly higher (P<0.01).Compared with LPS group,the level of SOD in LPS+MT1 group was significantly increased,however,the contents of MDA and MPO were decreased (P<0.05).The same trend was more prominent in LPS+MT2 group.Conclusions:MT can decrease the lung injury by reducing the inflammatory response and lipid peroxidation induced by LPS in rats with sepsis.The protective effect was positively associated with the dose of MT.
