The role and mechanism of FoxP3 in biological behavior of gastric cancer cells
10.12025/j.issn.1008-6358.2016.20160931
- VernacularTitle:叉头框蛋白3过表达对胃癌细胞生物学行为的影响
- Author:
Gui-Fen MA
1
;
Du-Yi PAN
;
Jian HE
;
Zhao-Chong ZENG
;
Shi-Yao CHEN
Author Information
1. 复旦大学附属中山医院放疗科
- Keywords:
Forkhead box protein;
gastric cancer;
gene function;
biological behavior
- From:
Chinese Journal of Clinical Medicine
2016;23(5):545-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role and mechanism of Forkhead box protein 3 (FoxP3)in biological behavior of gastric cancer (GC)cells.Methods:Invitro,the plasmid of FoxP3-shRNA was transfected into GC cells,and then the stably transfected cells were established by drug-screening and monoclone-selection.MTT assay was used to detect the growth and proliferation of GC cells.And cell cycle was detected by flow cytometry.MTT assay was used to measure the difference regarding sensitivity to chemotherapy drugs.Results:GC cell line which was stably transfected with FoxP3 gene,was established.GC cell line with up-regulation of FoxP3 gene,compared to the vector-transfected control,showed slower growth and proliferation rate,weaker ability of invasion (transmembrane cell counts:[203±42]cells/HPvs [891±100]cells/HP, P<0.05),and higher sensitivity to chemotherapy drugs(P<0.05).Conclusions:FoxP3 gene plays a role in inhibiting the growth of GC cells.
