Compatibility and ectopic bone formation of biphasic calcium phosphate ceramic
10.3760/cma.j.cn114453-20190806-00241
- VernacularTitle:双相生物钙磷陶瓷生物相容性及异位骨诱导的实验研究
- Author:
Xiaoshuang GUO
1
;
Haiyue JIANG
;
Guodong SONG
;
Xiaolei JIN
Author Information
1. 中国医学科学院北京协和医学院整形外科医院十六科 100144
- Keywords:
Hydroxyapatites;
Bone marrow;
Stem cells;
Biocompatibility;
Biphasic calcium phosphate ceramic;
Ectopic bone formation
- From:
Chinese Journal of Plastic Surgery
2021;37(1):94-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To manufacture one kind of biphasic calcium phosphate (BCP) ceramic by hydroxyapatite (HA) and β-tricalciumphosphate (β-TCP), and to further investigate its compatibility and its efficacy of ectopic bone formation.Methods:BCP was prepared with the ratio of HA and β-TCP at 6/4 using precipitation and H 2O 2 foaming method and then sintered at 1 100 ℃ for 3 hours. The chemical composition of BCP was investigated by X-ray diffraction(XRD). BMSCs were isolated from the bone marrow of Sprague-Dawley (SD) rat and seeded on BCP. The adhesion and morphology of BMSCs on BCP was observed under scanning electron microscope(SEM) and special staining. Cell proliferation was quantified by cell counting kit-8(CCK8) assay. Alkaline phosphatase(ALP)activity of BMSCs was measured by ALP assay kit. For further confirmation, the intramuscularly ectopic implantation models of Beagle were used, general observation, histological, and histomorphometric analyses were conducted at 4, 8, 12 weeks after implantation. Results:BCPs were successfully manufactured. XRD analysis showed the specific diffraction peaks of HA and β-TCP. SEM showed that the surface of the BCP ceramics was widely distributed with macropores and connections, and the pore walls were rough, and the micropores were evenly distributed in the macropores. Phalloidin and DAPI staining showed that the BMSCs extended and adhered to the surface of the material, and the shape gradually changed from irregularity to uniform long spindle. CCK8 method showed that although the cell viability decreased on the first day after coculture, on the third, fourth, fifth and seventh days, the cell viability gradually increased. The assay of alkaline phosphatase activity indicated that BMSCs cultured on the BCP could secrete more alkaline phosphatase on day 1 and 7 compared with the control group. BCP implanted in the muscle could generate osteoid/bone tissue at 8 weeks and 12 weeks, the number of osteoid/bone filled pores were 0.77±0.11, the percentage of osteoid/bone tissue inside the pores were 0.71±0.14.Conclusions:The BCP had a good biocompatibility and favorable efficacy of ectopic osteoinduction.
