Propofol Induces Neurotoxicity by Inhibiting tPA Release of Neuronal Synapse in Mouse Hippocampus
- VernacularTitle:丙泊酚通过抑制小鼠海马神经元突触释放组织纤溶酶原激活物引起神经毒性损伤
- Author:
Chao LIANG
1
;
Jing CANG
;
Zhanggang XUE
Author Information
1. 复旦大学附属中山医院麻醉科
- Keywords:
Propofol;
Developing neurons;
Neurotoxicity
- From:
Chinese Journal of Clinical Medicine
2016;23(1):67-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of propofol on the tPA release of hippocampal neurons in developing mice ,so as to investigate whether exogenous tPA could reverse the propofol‐induced neurotoxicity on developing neurons .Methods : The hippocampal neurons of developing mice cultured in vitro were divided into 4 groups(n= 20 each):control group(group C) , propofol group(group Pro) ,group tPA and propofol plus tPA group(group Pro+ tPA) .There was no specific treatment in group C .In group Pro ,propofol was added to the culture media with the final concentration of 5 ,10 ,30 μmol/L ,respectively , and the cells were then incubated for 1 h ,2 h ,3 h ,6 h ,respectively .In group tPA ,tPA was added to the culture media with the final concentration of 1 μmol/L ,and the cells were then incubated for 6 h .In group Pro+ tPA ,both propofol and tPA were added to the culture with the final concentration of 10μmol/L and 1μmol/L ,respectively ,and the cells were then incubated for 6 h .The neuron apoptosis rate and the expression level of actived‐caspase‐3 protein were detected in each group .Results:Compared with that in group C ,the apoptosis rate and the expression level of actived‐caspase‐3 protein in group Pro significantly increased (P< 0 .05) .The tPA level in group Pro was significantly higher than that in group C (P< 0 .05) . Compared with that in group Pro ,the apoptosis rate and the expression level of actived‐caspase‐3 protein in group Pro+ tPA significantly decreased (P< 0 .05) .Conclusions :Propofol induces neurotoxicity on hippocampal neurons of developing mice cultured in vitro by inhibiting neuronal tPA release .And exogenous tPA could partially reverse propofol‐induced neurotoxicity .
