Establishment of the Senescence Model of Rat Nucleus Pulposus Cell in Vitro
- VernacularTitle:大鼠髓核细胞体外衰老模型的建立
- Author:
Houlei NG WA
1
;
Wei LU
;
Ang Def LI
;
Lei DING
;
Jingping WU
Author Information
1. 复旦大学附属金山医院骨科
- Keywords:
Nucleus pulposus cells;
Senescence;
Tert-butyl hydroperoxide
- From:
Chinese Journal of Clinical Medicine
2016;23(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a senescence model of rat intervertebral disc nucleus pulposus cells .Methods :The nucleus pulposus cells ,which were extracted from rat intervertebral discs and cultured in complete medium ,were set as control group . The senescence model of nucleus pulposus cells in vitro (senescence model group) was established by additional culture for two hours on the basis of control group ,to which tert‐butyl hydroperoxide(t‐BHP) was added with a final concentration of 100μmol/L .The expression levels of senescence associated indexes ,such as caveolin‐1 and beta‐galactosidase (SA‐β‐gal) ,in two groups were assessed by Western blotting ,while the cell viability in two groups was detected by Cell Counting Kit‐8(CCK‐8) proliferation assay .Results :After two hours of t‐BHP function ,the expression levels of caveolin‐1and SA‐β‐gal in senescence model group were significantly higher than those in control group ,while the nucleus pulposus cells proliferation was slower and the cell viability was much lower .Conclusions :The senescence model of nucleus pulposus cells in vitro can be established successfully with the induction of t‐BHP ,and caveolin‐1 induces the senescence of nucleus pulposus cells during the process .
