The use of micro-plasma radio frequency technology to treat narrow band ultraviolet B induced guinea pigs skin pigmentation
10.3760/cma.j.cn114453-20200716-00430
- VernacularTitle:微等离子体射频技术对窄波中波紫外线诱导豚鼠皮肤色素沉着的影响
- Author:
Yan XU
1
;
Huizheng LI
;
Xingjian CHENG
;
Mo CAO
Author Information
1. 沧州市中心医院医学整形美容科 061001；河北医科大学第三医院烧伤整形科，石家庄 050051
- Keywords:
Ultraviolet irradiation;
Guinea pig;
Skin pigmentation;
Micro-plasma radio frequency
- From:
Chinese Journal of Plastic Surgery
2020;36(10):1106-1113
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study on the effects of micro-plasma radio frequency technology on guinea pig skin pigmentation animal model induced by narrow band ultraviolet B (NB-UVB).Methods:We selected ten healthy guinea pigs (common grade), removed the brown hair of the guinea pig and established UVB to induce the skin pigmentation model of guinea pig. The pigmentation zone was randomly divided into four groups: Group A was used as blank control with no treatment and Groups B , C and D were treated with micro-plasma radio frequency technology using different energy parameters. Group B treatment parameters were as follows: output power was 10 watts, and treatment energy was 0.06J. Group C treatment parameters were as follows: output power was 20 watts, and treatment energy was 0.12J. Group D treatment parameters were as follows: output power was 30 watts, and treatment energy was 0.18J. They were compared before treatment and at 2, 4, and 6 weeks after treatment, based on melanin cell staining (epidermis separation-dopa staining) and melanin granules dyeing method (Masson-Fontana). Immunohistochemical staining (c-kit protein) was used to observe the tyrosinase activity in the melanocytes, melanin granule percentage area of the skin area, and the change in melanin cell activity. SPSS13.0 statistical software was used for statistical analysis of the experimental data. The significance of the difference ( P< 0.05) was measured. Results:The results showed that micro-plasma radio frequency (RF) technology treatment could reduce the tyrosinase activity in the melanocytes, 2, 4 and 6 weeks after the end of treatment: Groups B, C and D were lower than group A, and the difference was statistically significant ( P<0.05), and as the treatment energy increased, the speed of reduction was increased. Micro-plasma radio frequency technology could reduce skin pigmentation and decrease the amount of epidermal melanin granules, 2 and 4 weeks after the end of treatment: Groups B, C and D were lower than Group A; 6 weeks after the end of treatment, only Group D was lower than Group A, with statistically significant differences ( P< 0.05). An increase in the energy of the micro-plasma radio frequency sped up the decline in the amount of epidermal melanin granules.The number of c-kit positive cells was not increased in this experiment. Two weeks after the end of treatment, the number of c-kit positive cells was higher than those of Groups B, C and D. There were no statistically significant differences in Groups B, C and D ( P<0.05). No c-kit positive cells were found in Groups A, B, C and D at 4 and 6 weeks after the irradiation. Conclusions:Micro-plasma radio frequency technology could accelerate the fading of guinea pig skin pigmentation. It is effective to improve pigmentation of guinea pig skin after NB-UVB irradiation.
