Study on Warburg effect in keloid fibroblasts
10.3760/cma.j.cn114453-20200220-00064
- VernacularTitle:瘢痕疙瘩成纤维细胞中Warburg效应的研究
- Author:
Zhiguo SU
1
;
Jincai FAN
;
Liqiang LIU
;
Hu JIAO
;
Hongbo CHEN
;
Yan ZENG
;
Yihua CHEN
Author Information
1. 中国医学科学院北京协和医学院整形外科医院整形九科 100144
- Keywords:
Keloid;
Glycometabolism;
Warburg effect;
Glycolysis
- From:
Chinese Journal of Plastic Surgery
2020;36(10):1100-1105
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether there is Warburg effect in keloid fibroblasts (KFs) , hypertrophic scar fibroblasts (HSFs) and atrophic scar fibroblasts (ASFs).Methods:KFs, HSFs, ASFs and NFs were isolated and cultured, respectively, in vitro. Glucose consumption and lactate production of the cells in each group were tested and compared. The mRNA expression of key glycolytic enzymes was detected, including hexokinase 2 (HK2), fructose-2, 6-biphosphatase 3 (PFKFB3), pyruvate kinase isoform M 2 (PKM2) and lactate dehydrogenase A (LDHA). In addition, the effect of 2-deoxy-D-glucose(2-DG) on the proliferation of KFs and NFs were detected and compared.Results:Glucose consumption and lactate production in KFs were 45.5% and 38.1% higher than those in NFs respectively ( P<0.01), while there was no statistical difference in glucose consumption and lactate production between ASFs and NFs, HSFs and NFs ( P>0.05). The mRNA expression of HK2, PKM2 and LDHA genes in KFs were 4.7, 2.7 and 1.8 times higher than those in NFs ( P<0.05), respectively, while the gene expressions in ASFs and HSFs had no statistical differences from those in NFs ( P>0.05). Under the action of 2-DG, the cell proliferation activity in KF group and NF group were reduced by 37.5% and 27.0%, respectively, indicating that the inhibition effect of glycolysis inhibitor on the proliferation activity of KFs was significantly higher than that of NFs. Conclusions:There is Warburg effect in KFs and there are no similar phenomena in HSFs and ASFs.
