MRI Tranking of Superparamagnetic Iron Oxide-Labeled Rabbit Adipose-Derived Stem Cells for Treatment of Carticular Carilage Defect
- VernacularTitle:MRI示踪超顺磁性氧化铁标记兔脂肪干细胞治疗关节软骨缺损的研究
- Author:
Kai LIU
1
;
Chunbo LI
;
Zenggan CHEN
;
Mengsu ZENG
;
Caixia FU
;
Caizhong CHEN
Author Information
1. 复旦大学附属中山医院放射科
- Keywords:
Magnetic resonance imaging;
Adipose-derived stem cells;
Superparamanetic iron;
Cell tracking
- From:
Chinese Journal of Clinical Medicine
2015;(5):589-595
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect dynamically in vivo superparamagnetic iron oxide‐labeled rabbit adipose‐derived stem cells (ADSCs) for treatment of carticular cartilage defect with MRI tracking technique .Methods:Rabbit ADSCs were firstly labeled with different concentrations of SPIO (0 μg/mL ,12 .5 μg/mL ,25 μg/mL ,50 μg/mL ,and 100 μg/mL) .The labeled ADSCs were stained with Prussian blue and the labeling rates were calculated .The activities of different concentration SPIO‐labeled ADSCs were determined by CCK‐8 . Different amounts of different concentration SPIO‐labeled ADSCs were dissolved homogeneously in cryovial with 1% agarose and T2*‐weighted MRI was used to evaluate their signal intensity in vitro .Then 50 μg/mL SPIO‐labeled ADSCs and unlabeled ADSCs were seeded in PLGA [Poly (lactic‐co‐glycolic acid )] scaffold , respectively .Following that ,the cells‐PLGA scaffold compounds were implanted in the rabbit (n=5) articular cartilage defect with average diameter 3 mm .At 1 ,4 ,8 ,and 12 weeks post‐operatively ,the change of implanted‐ADSCs and the repain of articular cartilage defect were dynamically detected by T2*‐weighted MRI .Results:Primarily cultured rabbit ADSCs presented fibroblast‐like appearance ,and ADSCs proliferated with aggregations after 7 days .Prussian blue staining showed amount of blue dense granules in cytoplasm .With the increasing of SPIO concentration , the count and region of blue dense granules increased .When SPIO concentration reached 50 μg/mL ,the labeling rate of ADSCs reached 100% .The measure of CCK‐8 showed that the activities of ADSCs decreased with the increasing of SPIO concentration .When the SPIO concentration was greater than 50 μg/mL ,the activities of ADSCs were obvious low (P<0 .05) .In vivo ,MRI showed postoperative 1 week and 4 weeks visible granules with low signal area ,and the signal strength was significantly lower than the control group .Until 8 weeks post‐surgery ,granules with low signal area gradually narrowed and signal strength was similar to the control group . SPIO‐labeled ADSCs lived with a normal division growth and performed normal cartilage articular .Conclusions:MRI combined SPIO is a potential method to dynamically detect the distribution ,growth and prognosis of cells in the body ,and can also be used to determine the respiration of articular cartilage defect .
