Effect of Up-Regulating miR-519d on Proliferation and Apoptosis of Laryngeal Squamous Cell Carcinoma
- VernacularTitle:上调 miR-519d 对喉鳞癌细胞增殖及凋亡的影响
- Author:
Na SHEN
1
;
Guangping BAI
;
Tianle GAO
;
Xinsheng HUANG
;
Jun LI
Author Information
1. 复旦大学附属中山医院耳鼻咽喉科
- Keywords:
Laryngeal squamous cell carcinoma;
miRNA-519d;
Signal transducer and activator of transcription 3;
Proliferation;
Apoptosis
- From:
Chinese Journal of Clinical Medicine
2015;(5):582-585
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of up‐regulating miR‐519d on proliferation and apoptosis of laryngeal squamous cell carcinoma .Methods:The expression of signal transducer and activator of transcription 3 (STAT3) and miR‐519d in human laryngeal carcinoma epithelium cell line Hep‐2 and human bronchial epithelium cell (HBE ) were detected by real‐time fluorescent quantitative polymerase chain reaction (RT‐PCR) and Western blotting .After up‐regulating miR‐519d expression in Hep‐2 cell line by plasmid transfection technique ,the proliferation and apoptosis of transfected cells and control cells was detected by proliferation and apoptosis assays .Results:STAT3 mRNA expression in Hep‐2 cells was significantly higher than that in HBE cells (P<0 .05) .However ,miR‐519d expression in Hep‐2 cells was significantly lower than that in HBE cells (P<0 .05) .The STAT3 mRNA expression in the transfected Hep‐2 cells decreased significantly .During 0 and 7 day of post‐transfection culture ,proliferation rate of transfected Hep‐2 cells was significantly lower than that of untransfected cells (P<0 .05) .After 24 h culture ,the apoptosis rate of miR‐519d transfected cells was (2 .8 ± 0 .15)% ,while that of control cells was (0 .92 ± 0 .09)% (P=0 .000 4) .After 72 h culture ,the apoptosis rate of miR‐519d transfected cells was (23 .06 ± 3 .52)% , while that of control cells was (23 .26 ± 2 .56)% (P=0 .965 5) .Conclusions:STAT3 shows high expression in Hep‐2 cell , while the related miR‐519d shows low expression .By up‐regulating miR‐519d ,STAT3 expression could be suppressed ,so as to suppress the proliferation of tumor cells and promote the apoptosis of tumor cells .
