Effect of In-Utero Exposure to Isoflurane on Apoptosis of Hippocampal Neuron in Fetal Rats
- VernacularTitle:异氟烷宫内暴露对胎鼠海马神经元凋亡的影响
- Author:
Zhiqiang LIU
1
;
Zhendong XU
;
Fuyi SHEN
;
Yueqi ZHANG
;
Haibing LI
Author Information
1. 同济大学附属第一妇婴保健院麻醉科
- Keywords:
Isoflurane;
In-utero exposure;
Apoptosis;
Hippocampal neuron
- From:
Chinese Journal of Clinical Medicine
2015;(5):579-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of in‐utero exposure to isoflurane on apoptosis of hippocampal neuron in fetal rats . Methods:SD rats at gestational day 19 were randomly assigned into the isoflurane anesthesia group(group I) and the control group(group C) .The rats in group I were exposed to 1 .3% isoflurane for 3 h ,while group C were exposed to air .The Apoptosis status of neurons in hippocampal CA1 region of fetal brains were observed by using TdT mediated dUTP nick end labeling(TUNEL) 0 h ,3 h ,and 9 h after exposure to isoflurane .Results:There were a certain amount of apoptotic neurons in hippocampal CA1 region in both group I and group C 0 h ,3 h ,and 9 h after the exposure .There were significant differences regarding apoptosis index(AI) 0 h and 3 h after the exposure between the two groups (P< 0 .05) .However ,there was no significant difference regarding the AI between the two groups 9 h after the exposure (P>0 .05) .Conclusions:The in‐utero exposure to 1 .3% isoflurane does not increase the apoptosis of neurons in hippocampal CA1 region of fetal rats .Furthermore , it may alleviate the apoptosis during the early period .The result suggests that fetus period may not be the vulnerable period for isoflurane‐induced neurotoxicity .
