Protective Effect of Diallyl Trisulfide on Hypoxia-Reoxygenation Injury of Endothelial Cells
- VernacularTitle:二烯丙基三硫化物对内皮细胞缺氧复氧损伤的保护作用
- Author:
Changfa GUO
1
;
Xiaotian SUN
;
Wenshuo WANG
;
Chunsheng WANG
Author Information
1. 复旦大学附属中山医院心外科
- Keywords:
Oxidative stress;
Mitochondrial membrane potential;
Proliferation;
Differentiation
- From:
Chinese Journal of Clinical Medicine
2015;(5):575-578
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of the protective effect of allicin ,of which the main composition is diallyl trisulfide ,on cardiovascular system .Methods:The endothelial cell model of hypoxia‐reoxygenation injury was the research object .Vitality change of endothelial cells was evaluated by using CCK8 .Confocal laser scanning microscope was used to detect the change of mitochondrial membrane potential in endothelial cells .Endothelial cells were planted onto matrigel so as to observe the difference regarding the ability of angiogenesis . Results: It showed that diallyl trisulfide performed the best protective effect at the concentration of 0 .2 μg/mL ,and its main mechanism based on maintaining the normal ability of proliferation and differentiation of endothelial cells after hypoxia‐reoxygenation process by reducing the depolarization level of mitochondrial membrane potential .Conclusions:Diallyl trisulfide alleviates the hypoxia‐reoxygenation injury of endothelial cells through the pathway involved with mitochondria .
