Effect of R302Q Mutation on Glycogen and Calcium Ion Homeostasis of Cardiomyocytes in PRKAG2 Cardiac ;Syndrome
- VernacularTitle:PRKAG2心脏综合征中 R302Q 突变对心肌细胞糖原和钙离子稳态的影响
- Author:
Yaping ZHANG
1
;
Yan YAN
;
Zhenju SONG
;
Chaoyang TONG
Author Information
1. 复旦大学附属中山医院急诊科
- Keywords:
PRKAG2 cardiac syndrome;
R302Q mutation;
Glycogen;
Calcium overload
- From:
Chinese Journal of Clinical Medicine
2015;(4):479-481
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of R302Q mutation on glycogen and calcium ion homeostasis of cardiomyocytes in PRKAG2 cardiac syndrome.Methods:The rat H9C2 cardiomyocytes were infected with adenovirus containing enhanced green fluorescent protein(EGFP),PRKAG2-WT,and PRKAG2-R302Q,meanwhile,normal control group was set up.After 24 h and 48 h,Periodic acid-Schiff(PAS)staining was used to observe cardiomyocyte glycogen storage,and the glycogen con-tent in cells was detected by enzyme-linked immunosorbent assay (ELISA).Meanwhile,Rohd-2/AM vital dye was used to car-ry out calcium ion staining,and then the signal differences among calcium stores were analyzed by flow cytometry.Results:There was no obvious difference regarding glycogen staining and content between EGFP group and control group.Glycogen staining and content was slightly enhanced in PRKAG2-WT group,while glycogen staining and content was obviously enhanced in PRKAG2-R302Q group.However,the calcium ion homeostasis in each group was not affected.Conclusions:R302Q muta-tion leads to intra cardiomyocyte glycogen accumulation in PRKAG2 cardiac syndrome.However,calcium ion homeostasis is not affected and there is no calcium overload.
