Change of Apoptosis of Human Nucleus Pulposus Cells Cultured in Vitro under Hypoxic Condition and Diffe-rent Hydrostatic Pressure
- VernacularTitle:低氧环境及不同流体静压下体外培养的人髓核细胞凋亡的变化
- Author:
Gui Yong WANG
1
;
Jiangtao LIU
;
Junchang XU
;
Zhengling ZHUANG
;
Tao LIU
;
Chang LIU
Author Information
1. 湖北医药学院附属襄阳医院骨科
- Keywords:
Intervertebral disc degeneration;
Apoptosis;
Cell hypoxia;
Hydrostatic pressure
- From:
Chinese Journal of Clinical Medicine
2015;(4):475-478
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the change of apoptosis of nucleus pulposus cells cultured in vitro under hypoxic condition and different hydrostatic pressure.Methods:The human degenerative nucleus pulposus cells were cultured in vitro and randomly divided into 2 groups according to hypoxic condition:the hypoxia group and the normal oxygen partial pressure group.While the nucleus pulposus cells were randomly divided into three groups according to different hydrostatic pressure:the control group without pressure,the 50 kPa pressure group and the 100 kPa pressure group.The change of apoptosis of nucleus pulpo-sus cells was detected by methyl thiazolyl tetrazolium(MTT)method and terminal dexynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)assay.Results:As compared with that in normal oxygen partial pressure group,the proportion of survival nucleus pulposus cells in hypoxic group increased,while the number of apoptotic cells decreased.Under different hy-drostatic pressure,there was no statistically significant difference regarding the apoptotic positive rate of nucleus pulposus cells between the 50 kPa pressure group and the control group (P >0.05 ).However,the apoptosis positive rate in the 100 kPa pressure group increased by 3 1 .65%,compared with that in the control group,and the difference was statistically significant (P <0.01).Conclusions:Hypoxic condition,as well as low pressure condition,can reduce the occurrence of apoptosis of nu-cleus pulposus cells.
