Thioether-Cyclized Helix B Peptide Alleviate Oxidative Stress Damage of HK-2 Cells by Inhibiting Endoplas-mic Reticulum Stress
- VernacularTitle:硫醚环化B螺旋肽通过抑制内质网应激减轻HK-2细胞的氧化应激损伤
- Author:
Long LI
1
;
Long ZHENG
;
Chao HU
;
Miao N LI
;
Yaqiu LONG
;
Ruiming RONG
Author Information
1. 复旦大学附属中山医院泌尿外科
- Keywords:
Thioether-cyclized helix B peptide;
Kidney;
Renal tubular epithelial cell;
Endoplasmic reticulum stress
- From:
Chinese Journal of Clinical Medicine
2015;(4):465-470
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the protective effect of thioether-cyclized helix B peptide (CHBP)on renal tubular epithelial cells and its mechanism by experiment in vitro.Methods:Human renal proximal tubular epithelial cell line HK-2 cells were cul-tured in vitro and divided into control group,H2 O2 group and CHBP group.Oxidative stress damage models were established by stimulation with 500 μmol/L H2 O2 for 4 h.In CHBP group,20 nmol/L CHBP was added to the culture medium 1 h prior to the establishment of oxidative stress model.After 4 h stimulation with 500 μmol/L H2 O2 ,cell viability,situation of apopto-sis,and severity of oxidative stress damage were measured.The expressions of Binding immunoglobulin protein(BiP),C/EBP homologous protein(CHOP),and the molecular in downstream NF-E2-related factor 2 (Nrf2)signaling pathway were detected by Western blotting and real-time PCR analysis,so as to evaluate the level of endoplasmic reticulum(ER)stress.Results:Oxi-dative stress induced cytotoxic injury could be alleviated by CHBP pretreatment.Kit-8 (CCK-8 )assay and TUNEL showed that,after CHBP pretreatment,cell viability increased,while cell apoptosis,oxidative stress and ER stress decreased.The levels of Nrf2 and heme oxygenase-1(HO-1)were significantly higher in CHBP group than those in H2 O2 group.Conclusions:CHBP pretreatment could alleviate oxidative stress damage of HK-2 cells,and the protective effect may be achieved by inhibi-ting ER stress and activating downstream Nrf2 signaling pathway.
