Expressions and Significance of Th1/Th2/Th17 Cytokines and Antimyocardial Antibodies in Mouse Dilated ;Cardiomyopathy
- VernacularTitle:Th1/Th2/Th17细胞因子及抗心肌抗体在小鼠扩张型心肌病中的表达及其意义
- Author:
Liping LI
1
;
Qing KONG
;
Wenying LAI
;
Xiaofen PAN
;
Weifeng WU
Author Information
1. 广西医科大学第一附属医院心内科
- Keywords:
Cytokine;
Antimyocardial antibody;
Dilated cardiomyopathy
- From:
Chinese Journal of Clinical Medicine
2015;(4):447-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expressions of Th1/Th2/Th1 7 cytokines and antimyocardial antibodies in mouse dilated cardiomyopathy (DCM),and explore their roles and significance in DCM pathogenesis.Methods:BALB/c mouse model of DCM was built with coxsackievirus(CVB3).Mice in control group were treated with phosphate-buffered saline (PBS)i.p. Mice were killed at the 24th week,and the fibrosis degree of myocardial tissue was observed by myocardial Masson’s staining. Expressions of Th1/Th2/Th1 7 cytokines in plasma were detected by using protein microarray.The mouse plasma levels of an-timyocardial antibodies such as adenine nucleotide translocator(ANT)antibody,β-myosin heavy chain(β-MHC)antibody,car-diac L-type calcium channel(CACH2),and anti-β1-adrenergic receptor(β1 AR)were measured by enzyme-linked immunosorbent assay (ELISA).Results:The result of myocardial pathological examination in DCM group fit the features of DCM 24 weeks af-ter virus infection.Compared with those in control group,expression levels of interleukin(IL)-2,IL-10,IL-13,IL-28 and TNF-αin DCM group decreased(P <0.05),however,the expression levels of IL-6,IL-1 7,IL-21 ,IL-22,IL-23 and TGF-βin-creased significantly(P <0.05).Meanwhile,compared with those in control group,the plasma levels of ANT andβ-MHC anti-bodies in DCM group increased significantly (P <0.05).Furthermore,positive correlation was found between level of IL-22 and level of anti-ANT according to correlative analysis(P <0.05 ).Conclusions:Immunologic derangement induced by imbal-ance of antimyocardial antibodies such as ANT and β-MHC,and Th1/Th2/Th1 7 cytokines such as IL-2、IL-10、IL-13、IL-28、TNF-α、IL-6、IL-1 7、IL-21 、IL-22、IL-23 and TGF-βmay play important roles in the pathogenesis of DCM.Furthermore,IL-22 may boost the production of ANT antibody.
