Diagnostic Value of Quantitative Detection of PAX1 Methylation for Cervical High Grade Precancerous Lesion
- VernacularTitle:定量检测 PAX1甲基化对于高级别宫颈癌前病变的诊断价值
- Author:
Xiao LIN
1
;
Jun XU
;
Ling XU
;
Ziyin XIA
;
Haoping ZHU
Author Information
1. 上海市闵行区中心医院妇产科
- Keywords:
Cervical precancerous lesions;
Pyrosequencing;
Paired boxed gene 1;
Methylation
- From:
Chinese Journal of Clinical Medicine
2015;(3):381-383
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical diagnostic value of quantitative detection of the paired‐boxed gene1 (PAX1 ) methylation for cervical high grade precancerous lesions .Methods:According to pathological diagnosis ,which was set as the gold standard ,122 patients infected with high risk subtype HPV were divided to the normal cervix group(group I ,n=39) ,the cervical low grade precancerous lesions group(group II ,n=40) ,and the cervical high grade precancerous lesions group(group III ,n=43) .The cervical tissue samples from each group were collected .The methylation level of PAX1 from each sample was measured by pyrosequencing and the statistical analysis was done .The diagnostic threshold of PAX1 methylation level for cer‐vical high‐grade precancerous lesions was verified by receiver operator characteristic(ROC) curve .Results:The PAX1 methyla‐tion level of group I ,group II ,group III was (4 .77 ± 2 .43)% ,(3 .75 ± 2 .36)% ,(13 .51 ± 5 .31)% ,respectively .And the differences among them were statistically significant (F=23 .13 ,P<0 .001) .The area under the ROC curve was 0 .73 .The diagnostic threshold of PAX1 methylation level for cervical high grade precancerous lesions was 4 .15% ,and its sensitivity and specificity was 89 .39% and 79 .15% ,respectively .Conclusions:Quantitative detection of PAX1 methylation level in cervical tissue by pyrosequencing could reach effective diagnosis for cervical precancerous lesions .
