Impact of miR-148a on the proliferation of gastric carcinoma MKN45 cells and its mechanism
10.3760/cma.j.issn.1671-0274.2014.10.019
- VernacularTitle:微小RNA-148a对胃癌细胞MKN45增殖能力的影响及机制研究
- Author:
Guangting QIU
1
;
Suihan WANG
;
Dapeng LI
;
Jikun LI
Author Information
1. 200080,上海交通大学附属第一人民医院普通外科
- Keywords:
Stomach neoplasms;
MicroRNA;
Cell proliferation;
Gene CDC25B
- From:
Chinese Journal of Gastrointestinal Surgery
2014;(10):1036-1039
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of miR-148a on cell proliferation of human gastric cancer cell lines MKN45. Methods Expression level of miR-148a was detected by qRT-PCR in the carcinoma tissues and the tissues adjacent to carcinoma of 60 patients with gastric cancer. Lentivirus packaging was used to establish MKN45 gastric cancer cell line expressing stable miR-148a as transfection group,and the untransfected MKN45 cell line as the control group. Gastric cancer MKN45 cell proliferation was detected by CCK8 method . The target gene of miR-148a was predicted by targetscan. Expression of target gene was examined by Western blotting. Result Expression of miR-148a in gastric cancer tissues of 54 cases (90.0%, 54/60) decreased, and among them, 37 tissue samples (61.7%, 37/60) decreased by 2 times. mir-148a expression in cancer tissues of patients with lymph node metastasis , vascular invasion and TNM stage Ⅲ-Ⅳ was down-regulated more obviously(P<0.05). From the third day after transfection, the growth of MKN45 cells was significantly inhibited (P<0.01). Gene CDC25B might be the target gene of miR-148a according to the results of targetscan. Western blot showed that CDC25B expression in transfection group was lower as compared to control group. Conclusion MiR-148a expression is down-regulated in gastric cancer tissues and inhibits gastric cancer cell proliferation. CDC25B may be the target gene of miR-148a that plays a role in tumor suppressor.
