Regulatory Effect of Constitutive Photomorphogenesis 9 Signalosome Subunit 6 on E6AP in Cervical Cancer and Its Clinical Significance
- VernacularTitle:宫颈癌中CSN6对E6AP的调节作用及其临床意义
- Author:
Chao WANG
1
;
Yu SONG
;
Qisang GUO
;
Yanmei LIU
;
Shujun GAO
Author Information
1. 复旦大学附属妇产科医院妇科
- Keywords:
Cervical cancer;
Human papilloma virus;
Infection;
Constitutive photomorphogenesis 9 signalosome;
E6AP
- From:
Chinese Journal of Clinical Medicine
2015;(3):272-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of constitutive photomorphogenesis 9 signalosome subunit 6(CSN6) in the regulation of E6AP in cervical cancer .Methods:CSN6 and E6AP genes were retrieved from The Cancer Genome Atlas(TCGA) ,and the correlation between them was analyzed .The effect of CSN6 on expression of E6AP and cytobiology was determined with trans‐fection of plasmids and Western blotting ,methyl thiazolyl tetrazolium(MTT) and flow cytometry(FCM) .Nude mice were sub‐cutaneously injected with Hela cell strains with different expression levels of CSN6 ,so as to set up the animal model .And the effect of CSN6 on growth of tumors was observed .Results:The database showed the overexpression and the mutation of CSN6 and E6AP in cancer .CSN6 could up‐regulate the expression of E6AP .The inhibition on CSN6 could decrease the expression level of E6AP and the proliferation of cells ,and induce the G0‐G1 arrest .Animal expreiment showed that knock‐out of CSN6 significantly inhibited the tumor growth ,decreasing E6AP level and increasing p53 level .Conclusions:CSN6 could contribute to the occurrence and progression of cervical cancer by up‐regulating the expression of E6AP .
