Phospho explorer antibody microarray analyze of congenital non-syndromic microtia
10.3760/cma.j.cn114453-20200301-00094
- VernacularTitle:先天性非综合征型小耳畸形的磷酸化抗体芯片分析
- Author:
Weiwei DONG
1
;
Leren HE
;
Haiyue JIANG
;
Yupeng SONG
;
Lin LIN
;
Qinghua YANG
Author Information
1. 中国医学科学院北京协和医学院整形外科医院整形七科 100144
- Keywords:
Congenital microtia;
Chondrogenesis;
Epigenomics;
phosphorylation
- From:
Chinese Journal of Plastic Surgery
2020;36(6):679-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the differential expression and significance of auricular cartilage protein phosphorylation profile in different degrees of development on the normal and the abnormal side of patients with congenital non-syndromic microtia.Methods:Collected 4 cases of unilateral congenital non-syndromic patients from March 2017 to March 2019 in Chinese Academy of Medical Sciences Plastic Surgery Hospital, including 4 pairs of matched normal auricle cartilage and residual auricle cartilage (2 males and 2 females, aged 6 years). Signal phosphorylation broad-spectrum screening antibody chip (PEX100) was used to screen the protein phosphorylation profiles of the cartilage on the normal and abnormal cartilages. The residual ear cartilage group was used as the experimental group, and the normal ear cartilage was used as the control group. The ratio of phosphorylation of the cartilage in the residual ear of patients was compared with that of the control group. Residual ear cartilage protein phosphorylation / normal ear cartilage protein phosphorylation ratio ≥2.0 indicates an up-regulated protein, and ≤0.5 indicates a down-regulated protein. The differential proteins obtained from the experiments were analyzed using String database and KEGG(Kyoto Encyclopedia of Genes and Genomes) PATHWAY databases.Results:The experimental/control group (residual ear cartilage/ normal ear cartilage) had a protein phosphorylation ratio of FC ≥ 2 and ≤ 0.5 with 110 proteins. Among them, 102 were up-regulation and 8 were down-regulation. The KEGG database was used to perform signal pathway enrichment analysis of proteins showing differences in phosphorylation levels. The screened differential proteins were enriched in 20 signaling pathways including PI3K-AKT, Focal adhension singaling pathway except the classic signaling pathways and the list of differentially phosphorylated proteins was imported into the STRING database to map the protein interactions network.Conclusions:There is a significant difference in the phosphorylation of proteins between normal auricle cartilage and residual auricle cartilage. Differentially phosphorylated proteins are enriched in signal pathways such as PI3K-AKT and focal adhesion, which suggests that the cause of the difference in development of bilateral ear cartilage may be related to ear cartilage proliferation, adhesion, migration and other activities during the embryonic period.
