Non-targeted metabonomics study on congenital microtia based on ear cartilage
10.3760/cma.j.cnZHZXWKZZ-2018-1115-00462
- VernacularTitle:先天性小耳畸形差异性代谢物质及代谢通路的非靶向筛查
- Author:
Jinxiu YANG
1
;
Leren HE
;
Lin LIN
;
Yongzhen WANG
;
Ye ZHANG
;
Zheng YANG
;
Wenfang DONG
;
Chen YANG
;
Qinhao GU
Author Information
1. 中国医学科学院北京协和医学院整形外科医院整形七科 100144（现在解放军总医院烧伤整形科，北京 100853）
- Keywords:
Microtia;
Metabonomics;
Pathogenesis;
Residual ear cartilage tissue
- From:
Chinese Journal of Plastic Surgery
2020;36(5):507-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Based on ear cartilage of congenital microtia, we tried to find the different metabolites and variational metabolic pathways on molecular level by gas chromatography time-of-flight mass spectrometry (GC-TOFMS).Methods:Patients with microtia were collected from June 2017 to January 2018. During operation, the ear cartilage tissue was collected and labeled, and the case group was residual ear cartilage tissue of the patients with microtia, with 18 cases. The control group was normal ear cartilage tissue, total 18 cases. After the sample pretreatment, based on the technique of GC-TOFMS and the software of XploreMET, the differential metabolites in the residual ear cartilage of microtia were analyzed by orthogonal partial least square discriminant analysis (OPLS-DA) and its variable importance projection (VIP) and U test statistical method. Compared with the metabolite database, the metabolic pathway enrichment analysis (MPEA) was used to screen the most related metabolic pathways. Results:According to the multidimensional statistical analysis, it is different in the metabolites of ear cartilage between the two groups. According to the verification of single dimensional statistical analysis, 14 specific biomarkers were identified, and their P value was less than 0.05. According to the metabolic pathway enrichment analysis (MPEA), 3 metabolic pathways had statistically significant difference, including arginine metabolism, taurine and the taurine metabolism, pantothenate and CoA metabolism, beta-alanine metabolism, glycerophospholipid metabolism, P <0.05. Conclusions:Arginine, taurine, L-cysteine and pantothenic acid may have an association with microtia, which proved that it is feasible to study the pathogenesis of microtia by metabonomics.
