Resveratrol Reduces Cardiac Ischemia-Reperfusion Injury of Diabetic Rats by Promoting Autophagy in Experimental Study
- VernacularTitle:白藜芦醇通过促进自噬减轻糖尿病大鼠心肌缺血再灌注损伤的实验研究
- Author:
Jiajia SHEN
1
;
Ying HUANG
;
Weigang QI
;
Jinghua TAN
;
Daifu ZHANG
Author Information
1. 上海市浦东新区人民医院心内科
- Keywords:
Resveratrol;
Diabetic model;
Ischemia-reperfusion injury model;
Autophagy;
Rats
- From:
Chinese Journal of Clinical Medicine
2015;(2):151-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective mechanism of resveratrol in reducing cardiac ischemia‐reperfusion injury (I/R) of diabetic rats and its effect on autophagy of cardiomyocytes .Methods:Streptozotocin(STZ)‐induced diabetic rat model was constructed and 35 diabetic model rats were randomly divided into five groups ,the sham group ,the resveratrol non‐surgery group ,the I/R group ,the resveratrol treatment group ,and the resveratrol+insulin group ,with 7 rats in each .Then I/R model was built by ligating‐relaxing the left coronary .The change of myocardial infarction size was observed by methylene blue stalning . Autophagic vacuoles were observed with transmission electron microscope (TEM ) . The expression of microtubule‐associated protein 1 light chaln 3(LC3)‐Ⅰ and Ⅱ ,autophagy related gene 5(Atg5) ,Beclin‐1 ,as well as the expression and phosphorylation levels of AMP‐activated protein kinase(AMPK) and mammalian target of rapamycin(mTOR) , were detected by Western blotting .Results:The infarction size in resveratrol treatment group and resveratrol+ insulin group was significantly smaller than that in I/R group(P<0 .05) .TEM showed that in resveratrol treatment group and resveratrol+insulin group ,autophagy of cardiomyocytes could be promoted and the structure of cardiomyocytes could be improved ,when I/R of occurred .The expression levels of LC3‐Ⅱ ,Atg5 ,Beclin‐1 in I/R group were significantly lower than those in sham group (P<0 .05) ,whereas the change of expression levels of LC3‐Ⅰ had no significant difference(P> 0 .05) .Meanwhile ,the expression levels of Atg5 and Beclin‐1 in resveratrol treatment group and resveratrol+ insulin group were significantly higher than those in I/R group ,and the expression level of Beclin‐1 in resveratrol+insulin group was higher than that in resveratrol treatment group(P< 0 .05) .There was no significant difference in the expression level of AMPK and mTOR among the 5 groups(P> 0 .05) .However ,the expression level of p‐AMPK was significantly higher in resveratrol treatment group and resveratrol+insulin group than that in I/R group ,and the expression level of p‐mTOR was significantly lower in resveratrol treatment group and resveratrol+ insulin group than that in I/R group ( P< 0 .05 ) .Conclusions:Resveratrol may protect cardiomyocytes of diabetic rats by activating AMPK pathway ,inhibiting mTOR pathway ,increasing the expression of LC3‐Ⅱ , Atg5 and Beclin‐1 ,promoting autophagy ,suppressing I/R ,and reducing infarction size .
