Preparation of Clindamycin Loaded Chitosan Sustained-Release Microspheres and Drug Release Study in Vitro
- VernacularTitle:克林霉素几丁糖缓释微球的制备及其体外缓释作用的研究
- Author:
Ji XU
1
;
Deqiang MENG
;
Dechang LIU
Author Information
1. 复旦大学附属金山医院骨科
- Keywords:
Clindamycin;
Chitosan;
Microspheres;
Sustained-release effect;
Bacteriostasis
- From:
Chinese Journal of Clinical Medicine
2015;(2):147-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare clindamycin loaded chitosan sustalned‐release microspheres ,determine drug loading and drug encapsulation efficiency of the microspheres ,and observe the effect of sustalned‐release and bacteriostasis in vitro .Methods:The microspheres were prepared by emulsified chemical cross‐linking method . The morphology and particle size of microspheres were observed by transmission electron microscopy and laser diffraction particle size analyzer .The drug loading and drug encapsulation efficiency of clindamycin loaded chitosan sustalned‐release microspheres were tested by UV spectrophotometry method .The effect of drug sustalned‐release and bacteriostasis in vitro was studied by establishing release system in vitro .The bacteriostasis of microspheres in vitro was observed by plotting the area‐time curve of inhibition ring . Results:The size of clindamycin loaded chitosan sustalned‐release microspheres was uniform ,while the dispersion was good . The average particle size of the microspheres was (153 .2 ± 13 .7)μm .The average drug loading was(39 .8 ± 1 .6)% .The mean encapsulation efficiency was (79 .6 ± 3 .5)% .The cumulative release percentage of clindamycin loaded chitosan sustalned‐release microspheres in pH 7 .4 phosphate buffer saline(PBS) was 16 .5% at the first hour ,21 .6% at the second hour ,33 .2%at the third hour and 53 .7% at the twenty‐fourth hour .The cumulative release percentage of clindamycin control was 99 .6% at the third hour .The microspheres still had obvious bacteriostasis after 3 days’ release ,as the bacteriostasis time area curve of the drug loaded microsphere showed .Conclusions:The encapsulation efficiency of clindamycin loaded chitosan sustalned‐release microspheres was good ,so that the sustalned‐release effect and bacteriostsis in vitro was also good .
