Isoflurane Induces the Apoptosis of Mouse Neural Stem Cells by Inhibiting Notch-1 Pathway
- VernacularTitle:异氟醚抑制 Notch-1通路诱导小鼠神经干细胞凋亡
- Author:
Lengchen HOU
1
;
Fuqing LIN
;
Shukun FU
;
Ya FENG
;
Te LIU
Author Information
1. 同济大学附属第十人民医院麻醉科
- Keywords:
Isoflurane;
Neural stem cells;
Notch-1 pathway;
Apoptosis
- From:
Chinese Journal of Clinical Medicine
2015;(2):122-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of isoflurane on neural stem cells and its possible mechanism .Methods:Mouse neural stem cells ,isolated and cultured in vitro ,were divided into the control group ,the phosphate‐buffered saline (PBS) group and the isoflurane group .The control group was not intervened .The isoflurane group exposed to isoflurane for 24 h ,while the PBS group exposed to the same amount of PBS .Then CCK‐8 assay was used to detect the cell proliferation rate . Real‐time quantitative polymerase chaln reaction was used to detect the mRNA levels of apoptosis‐related genes .Expression of apoptosis‐related protein was measured with Western blotting .Annexin V‐FITC/PI kit was used to assess cell apoptosis . Results:Compared with those in the control group and the PBS group ,cell proliferation rate ,as well as the mRNA and protein levels of Notch‐1 ,CBF‐1 and Hes‐1 ,in the isoflurane group ,decreased significantly .There were more apoptotic cells in the isoflurane group than in the control group and the PBS group .Conclusions:Isoflurane can induce the apoptosis of mouse neural stem cells ,and the process is closely related to the inhibition of Notch‐1 pathway .
