Effects of Vitrification and Program Freezing on the Follicular Viability of Human Ovarian Tissues
- VernacularTitle:玻璃化冷冻及程序冷冻对人卵巢组织卵泡活性的影响
- Author:
Liying LIU
1
;
Wenyu QU
;
Li JIANG
;
Xiaoli ZHANG
;
Xiaoli LIU
;
Gang KONG
Author Information
1. 辽宁省沈阳市妇婴医院生殖中心
- Keywords:
Vitrification;
Program freezing;
Human ovarian tissues;
Hormone releasing assay;
Ultrastructure;
T ransplantation
- From:
Chinese Journal of Clinical Medicine
2015;(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the effect of vitrification and program freezing on the follicular viability of human ovarian tissues .Methods :Ovarian tissues from 15 patients were transported to the laboratory within 20 min after excision .Pieces of ovarian tissues (1 mm × 5 mm × 5 mm)were randomly divided into fresh group ,vitrification group and program freezing group . Optimal experimental method was used in vitrification group and program freezing group .The fresh and resuscitated tissues were cultivated in vitro ,and the follicular morphology was analyzed by using light and electron microscope .The viability of ovarian tissues resuscitated from freezing was detected by hormones releasing assay and allograft assay ,and then compared with that of fresh group .Results:Morphologically normal and abnormal primordial and primary follicles could be seen in resuscitated freezing group and fresh group .The rate of morphologically normal follicles in fresh group was significantly higher than those in vitrification group and program freezing group (P<0 .05) .The rate of morphologically normal follicles was slightly higher in program freezing group than that in vitrification group (P>0 .05) .Primordial and primary follicles with normal and abnormal ultrastructure could be seen in all the three groups .Ovarian cortical pieces secreted estradiol and progesterone continuously after in vitro culture .Hormone levels in fresh group were higher than those in vitrification group and program freezing group (P>0 .05) .Allograft assay showed that there were more graft recovery number and higher follicular density in program freezing group than those in vitrification group .Conclusions :Follicle has higher potentiality of development in program freezing human ovarian tissues ,so program freezing is more promising than vitrification in application .
