Study on the Expression of High Mobility Group Box-1 Protein in Rat Model of Ventilator-Induced Lung Injury
- VernacularTitle:高迁移率族蛋白1在呼吸机相关性肺损伤大鼠中的表达变化
- Author:
Jiefei MA
1
;
Yizhou HE
;
Zhe LUO
;
Guowei TU
Author Information
1. 复旦大学附属中山医院重症医学科
- Keywords:
Ventilator-induced lung injury;
High mobility group box-1 protein;
Ethyl pyruvate
- From:
Chinese Journal of Clinical Medicine
2015;(1):29-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of high mobility group box‐1 (HMGB1) in rat model of ventilator‐induced lung injury (VILI) .Methods :Twenty‐four healthy adult SD rats were randomly divided into three groups ,with 8 rats in each group .The control group:autonomous respiration .The large tidal volume group :tidal volume(TV)= 30 mL/kg .The ethyl pyruvate(EP) intervention group :EP 100 mg/kg via the caudal vein and the same ventilation mode as the large tidal volume group .The levels of HMGB1 and inflammatory factors in serum and bronchoalveolar lavage fluid (BALF)and lung tissues ,as well as in the culture medium of alveolar macrophages before and after mechanical stretching ,were detected with Western blot‐ting ,RT‐PCR and ELISA .Results:The levels of HMGB1 in serum ,BALF and lung tissues of the large tidal volume group were higher than those of the control group .HMGB1 in the culture medium of alveolar macrophages was increased under me‐chanical stretching .The expression of HMGB1 decreased ,and the proteins related to signal pathway of apoptosis as Caspase3 , Caspase9 and PARP increased ,in lung tissues of rat with VILI after EP intervention .Conclusions :The expression of HMGB1 increases in rat model of VILI ,which may be related with the effect of mechanical stretching on alveolar macrophages .EP intervention had certain protective effect on lung tissues with VILI .
