Effects of Gender and Age on the Expression of Interferon Stimulated Gene in the Peripheral Blood of Patients with Chronic Hepatitis C
- VernacularTitle:性别和年龄对慢性丙型肝炎患者外周血中干扰素诱导基因表达的影响
- Author:
Zhili NIU
1
;
Ping′an ZHANG
;
Yongqing TONG
Author Information
1. 武汉大学人民医院检验科
- Keywords:
Chronic hepatitis C;
Peripheral blood mononuclear cells;
Interferon stimulated gene
- From:
Chinese Journal of Clinical Medicine
2015;(1):25-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mRNA expression of interferon‐induced protein with tetratricopeptide repeats 1 (IFIT1) and IFIT3 in the peripheral blood mononuclear cells (PBMC) in patients with chronic hepatitis C (CHC) with different genders and ages .Methods :Anticoagulant whole blood was collected from 215 patients with CHC and 79 healthy individuals . The mRNA expression levels of IFIT1 and IFIT3 were detected by real‐time quantitative PCR .Alanine aminotransferase(ALT) and aspartate aminotransferase (AST ) were detected by automatic biochemical analyzer . HCV‐RNA was detected with fluorescent probe .Results:Expression levels of IFIT1 and IFIT3 in PBMC of patients with CHC were significantly different from those in healthy people (t=22 .35 , P< 0 .05 ;t= 22 .75 , P< 0 .05) .There were statistically significant differences in ALT and AST between patients with CHC and healthy people (t=34 .38 , P<0 .05 ;t=21 .38 , P<0 .05) .The correlation analysis of gene expression levels of IFIT 1 and IFIT3 in PBMC with age ,gender ,liver function and HCV load in CHC patients revealed that the mRNA expression levels of IFIT 1 and IFIT3 were positively correlated with age (r=0 .395 , P<0 .05 ;r=0 .482 ,P<0 .05) and gender(r=0 .453 ,P<0 .05 ;r=0 .358 ,P<0 .05) .Conclusions :The expressions of IFIT1 and IFIT3 in PBMC are correlated with gender and age in CHC patients .
