Expression of E-cadherin in Rat Model of Hepatic Carcinoma during Transarterial Embolization and Its Rela-tionship with Pulmonary Metastasis
- VernacularTitle:E-cadherin 在经动脉栓塞治疗肝癌时的表达及其与肝癌肺转移的关系
- Author:
Wei ZHANG
1
;
Sheng QIAN
;
Xudong QU
;
Liang ZHU
;
Bo ZHOU
;
Guowei YANG
;
Jianhua WANG
;
Rong LIU
;
Zhiping YAN
Author Information
1. 复旦大学附属中山医院介入治疗科
- Keywords:
Hepatic carcinoma;
Embolization;
Rat;
Pulmonary metastasis;
E-cadherin
- From:
Chinese Journal of Clinical Medicine
2015;(1):10-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To conduct transarterial embolization (TAE) in rat model of hepatic carcinoma and to investigate the effect of embolization on pulmonary metastasis and expression of E‐cadherin .Methods :Forty Buffalo rats were chosen and rat models of hepatic carcinoma with stable expression of green fluorescent protein (EGFP) were established .Rats were randomly divided into four groups with 10 rats in each group .The control group(Group A) were treated with 0 .2 mL of 0 .9% sodium chloride solution by gastroduodenal artery retrograde catheterization .The hepatic artery ligation group(Group B) were treated with suture ligation after separation of hepatic artery .The low dose embolization group(Group C) were treated with lipiodol ul‐trafluide(0 .2 mL/kg) by gastroduodenal artery retrograde catheterization .The high dose embolization group(Group D) were treated with lipiodol fluide(0 .4 mL/kg) by gastroduodenal artery retrograde catheterization .Body weights ,tumor volumes were evaluated after operation .At 21 d after operation ,the rats were killed and the samples of liver and lung were collected .The changes of tumor growth and the situations of pulmonary metastasis were evaluated .The changes in E‐cadherin mRNA and protein expression levels were detected by real‐time fluorescent quantitative PCR ,Western blotting and immunohistochemistry . Results:At 21 d after operation ,the hepatic tumor growth was obviously inhibited in Group D ,and the tumor growth in Group B or Group C was also inhibited while compared with that in Group A (P< 0 .01) .The number of pulmonary metastasis in Group D was lower than those in the other groups(P<0 .01) .The levels of E‐cadherin mRNA and protein in Group D were higher than those in the other groups and the differences were statistically significant (P<0 .01) .The rate of E‐cadherin‐posi‐tive cells in Group D were higher than those in the other groups according to immunohistochemistry ,and the differences were statistically significant(P<0 .01) .Conclusions :TAE ,in the treatment of hepatic carcinoma on the rat models ,can promote E‐cadherin expression ,and high dose lipiodol embolization can inhibit tumor grow th and reduce pulmonary metastasis .
