Effects of Sorafentib on the Expression of SDF1-α in Hepatocellular Carcinoma Tissues and Pericarcinoma Liver Tissues
- VernacularTitle:索拉非尼对肝癌组织和癌旁肝组织中SD F1-α表达的影响
- Author:
Lu LU
1
;
Wenwei ZHU
;
Jinhong CHEN
;
Huliang JIA
Author Information
1. 复旦大学附属华山医院普外科
- Keywords:
Hepatocellular carcinoma;
Sorafenib;
Stromal-derived factor 1-α;
Invasion and metastasis
- From:
Chinese Journal of Clinical Medicine
2015;(1):5-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of the increased invasive and metastatic potential of hepatocellular carcinoma induced by sorafenib ,by studying the expression of stromal‐derived factor 1‐α(SDF 1‐α) .Methods :BALB/c nude mice models of hepatocellular carcinoma in situ were established with cell line HCCLM 3 .The mice were divided into sorafenib group and control group ,with 6 mice in each group .Sorafenib was administered intragastrically [30 mg/(kg · d)] in sorafenib group from 2 weeks after model had been established ,while 0 .9% sodium chloride solution was given in the control group .Mice were killed after treating for 4 weeks .The mRNA expressions of inflammation‐related factors in hepatocellular carcinoma tissues and pericarcinoma liver tissues of the two groups were detected by RT‐PCR .The protein expression of SDF1‐α in hepatocellular carcinoma tissues and pericarcinoma liver tissues was measured by immunohistochemistry .The protein expression of SDF1‐αin pericarcinoma liver tissues was also detected by Western blotting .ELISA was performed to detect the SDF1‐αconcentration in peripheral blood .Results:The mRNA expression of murine SDF1‐αin hepatocellular carcinoma tissues and pericarcinoma liver tissues was significantly increased by sorafenib therapy .The results of immunohistochemistry and Western blotting showed that protein expression level of SDF1‐α in hepatocellular carcinoma tissues and pericarcinoma liver tissues was significantly up‐regulated by sorafenib .The results of ELISA assay showed that the concentration of murine SDF1‐αin peripheral blood was increased by sorafenib .Conclusions :The expression of SDF1‐α in hepatocellular carcinoma tissues and pericarcinoma liver tissues can be up‐regulated by sorafenib therapy .
