Correlation and clinical significance of expressions of HIF-1αand Sema4D in colorectal carcinoma tissues
10.3760/cma.j.issn.1671-0274.2014.04.021
- VernacularTitle:低氧诱导因子-1α和轴突导向因子4D在结直肠癌组织中的表达及临床意义
- Author:
Linjun MU
1
;
Jinshen WANG
;
Xiaobo GUO
;
Sheng ZHENG
;
Keshu SHAN
;
Changqing JING
;
Leping LI
Author Information
1. 山东大学附属省立医院胃肠外科
- Keywords:
Colorectal neoplasms;
Immunohistochemical staining;
Hypoxia-inducible factor-1 alpha;
Semaphorin 4D;
Prognosis
- From:
Chinese Journal of Gastrointestinal Surgery
2014;(4):388-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the expressions of hypoxia-inducible factor 1 alpha (HIF-1α) and Semaphorin 4D (Sema4D) in colorectal carcinoma and normal colorectal tissues, and to investigate their correlation and clinical significance. Methods The expressions of HIF-1α and Sema4D were examined in 86 cases of colorectal carcinoma and 52 normal colorectal tissues by SP immunohistochemical staining. Correlation between these two expressions and association of the expressions with clinicopathological characters and prognosis were analyzed. Results The positive rates of HIF-1α and Sema4D protein in colorectal carcinoma tissues were significantly higher than those in normal colorectal tissues (58.1% vs. 7.7%, χ2=34.624,P<0.01; 60.5% vs. 11.5%, χ2=31.839, P<0.01). HIF-1α and Sema4D protein expressions were closely associated with colorectal carcinoma histological types (P=0.003,P=0.010), TNM staging (P=0.003,P=0.017) and lymphatic metastasis (P=0.003,P=0.020), and a significant correlation was observed between the expressions of HIF-1α and Sema4D protein (r=0.567, P<0.01). The 5-year overall survival rate was 37%. Univariate analysis showed that 5-year survival rates of patients with positive and negative HIF-1α protein expression were 24% and 56%(P=0.003), and those with positive and negative Sema4D protein expression were 23% and 59%(P=0.001). Multivariate Cox analysis showed that expression of Sema4D was an independent prognostic factor of colorectal cancer patients(P=0.026), while expression of HIF-1α was not(P=0.501). Conclusion Combined detection of HIF-1α and Sema4D has the potential to predict the development trend of colorectal carcinoma and prognosis of patients.
