The hyaluronidase enzymolysis experiment research of hyaluronic acid in the arteries
10.3760/cma.j.issn.1009-4598.2017.s1.024
- VernacularTitle:透明质酸酶降解动脉内透明质酸的实验研究
- Author:
Lei ZHANG
1
,
2
;
Ce ZHANG
;
Linggang LIANG
;
Ye ZHAO
;
Yi SUN
;
Sufan WU
Author Information
1. 310014 杭州,浙江省人民医院整形外科
2. 浙江中医药大学
- Keywords:
Hyaluronic acid;
Filler;
Cosmetic injection;
Blindness;
Ophthalmic artery embolism
- From:
Chinese Journal of Plastic Surgery
2017;33(z1):115-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether the hyaluronidase could infiltrate through the wall of blood vessel and further dissolve the hyaluronic acid within the arteries .Methods Rabbit abdominal aorta in 2 mm of diameter was filled with hyaluronic acid , which was then immersed in a high concentration of hyaluronidase for 90 minutes.The treated hyaluronic acid within artery was observed using optical microscope and laser diffraction particle size analyzer , to detect the change of particle size;Immunohistochemical study of blood vessels was performed to detect whether hyaluronidase penetrate the walls of the blood vessels;Hyaluronidase was injected into the vessel and immersed in saline , permeability of which was detected using ninhydrin dyeing and enzyme assay test .In addition, three patients of vision defects caused by hyaluronic acid injection were observed , two cases of which had received retrobulbar injection of hyaluronidase .Results After immersed within hyaluronidase , the particle sizes of hyaluronic acid had no changes compared with control group ( immersed within saline ) under the analysis of the laser diffraction analyzer and optical microscope .In immunohistochemical experiment , hyaluronidase was only detected in the outside wall of artery .Clinical cases showed that the retrobulbar injection of hyaluronidase could not decrease the symptoms of vision loss .Conclusions To an artery in a diameter of 2 mm, hyaluronidase could not penetrate through the arterial wall to dissolve the hyaluronic acid within the vessel . Thus, for the cases of ophthalmic artery embolism , retrobulbar injection of hyaluronidase may not be a logical solution .
