Hepatocyte Growth Factor Enhances the Proliferation of Human Gastric Cancer Cell Line AGS
- VernacularTitle:肝细胞生长因子对人胃癌细胞株 AGS 增殖的促进作用
- Author:
Yuexin LING
1
;
Saixiong TONG
Author Information
1. 复旦大学附属中山医院普外科
- Keywords:
Hepatocyte growth factor;
Human gastric cancer cells;
Proliferation
- From:
Chinese Journal of Clinical Medicine
2014;(5):529-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Hepatocyte growth factor (HGF)is a polypeptide growth factor with effects on the cell proliferation, motility,and morphogenesis.The purpose of this research was to explore the effect of HGF on the proliferation of human gas-tric cancer cell line AGS.Methods:Human gastric cancer cell line AGS was cultured in vitro.AGS cells were treated with 25, 50 ,75 and 100ng/mL of HGF for 24 h,48 h and 72 h,respectively.Cell proliferation was measured by CCK-8 assay.Results:CCK-8 assay result showed that compared with the control,all of the four concentrations of HGF could increase the prolifera-tion of AGS cells.With the increase of HGF concentration and treating time,the proliferation activity of AGS cells was the up-ward trend in a dose-and time-dependent manner (t=3.89,5.28,9.73,11.00,P <0.01).Conclusions:HGF can significant-ly promote the proliferation of human gastric cancer AGS cells.
