Effect of Electrical Stimulation of Vagus Nerve on the Expression of Calcium-Binding Protein S100A8 in Septic Shock Rats
- VernacularTitle:电刺激迷走神经对感染性休克大鼠钙结合蛋白 S100A8表达的影响
- Author:
Ming LEI
1
;
Kailiang XU
;
Longshu ZHAO
;
Yulong YAO
;
Xiumei CHEN
Author Information
1. 上海市第七人民医院重症医学科
- Keywords:
Septic shock;
Vagus nerve;
Electrical stimulation;
Calcium-binding protein S100A8;
Model;
Rat
- From:
Chinese Journal of Clinical Medicine
2014;(5):526-528
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish septic shock rat model and to investigate the effect of electrical stimulation of vagus nerve on the expression level of plasma calcium-binding protein S100A8 in septic shock rats.Methods:Thirty male adult rats were per-formed cecal ligation and puncture (CLP)to establish septic shock model and then randomly divided into six group:Group I, sham electric acupuncture and normal;Group II,electric acupuncture and normal;Group III,sham electric acupuncture and CLP;Group IV,electric acupuncture and CLP;Group V,vagotomy,sham electric acupuncture and CLP;Group VI,vagoto-my,electric acupuncture and CLP.In Group III,IV,V and VI,indwelling catheter was placed in the common carotid artery for monitoring mean arterial blood pressure (MAP)continuously.Plasma calcium-binding protein S100A8 was determined by ELISA.Results:In groups with CLP,MAP gradually decreased,and serum S100A8 level at postoperative 12 h significantly increased (P <0.01).Compared to that in groups with CLP,the decreased extent of MAP reduced in rats treated with electri-cal stimulation (P <0.01).Moreover,the decreased extent of MAP reduced and the level of plasma calcium-binding protein S100A8 significantly decreased in Group VI,compared to that in Group V (P <0.01).Conclusions:The electrical stimulation of vagus nerve can alleviate gradual decrease of MAP which was caused by CLP induced septic shock in rats and reduce the level of plasma calcium-binding protein S100A8,so that it contributes to anti-shock treatment as supplementary role.
