Protective Effect of Fasudil on the Striated Muscle in Rats with Acute Organophosphorus Pesticides Poisoning
- VernacularTitle:法舒地尔对急性有机磷中毒大鼠横纹肌的保护作用
- Author:
Luojia TANG
1
;
Chenling YAO
;
Chen ZHANG
Author Information
1. 复旦大学附属中山医院急诊科
- Keywords:
Dichlorvos;
Acute organophosphorus pesticides poisoning;
Fasudil;
Striated muscle
- From:
Chinese Journal of Clinical Medicine
2014;(5):517-519
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of fasudil on the striated muscle in rats with acute organophosphorus pesticides poisoning(AOPP).Methods:The AOPP model was established by peritoneal injection of dichlorvos (DDVP).Rats were randomly divided into control group(n=8),poisoned group(n=8)and pretreated group(n=8).All rats in the 3 groups were supported with breathing machine connected with the tube from incision of trachea.The control group was peritoneally in-jected 0.9% sodium chloride solution (0.4 mL).The poisoned group and the pretreated group were peritoneally injected DDVP (30 mg/kg).The pretreated group was peritoneally injected fasudil(10 mg/kg)30 min before anesthesia.Serum level of cardiac troponin T(cTnT)was measured by using autonomic biochemical analyzer,and creatine kinase isoenzyme MM(CK-MM)and lactate dehydrogenase isoenzyme M(LDH-M)were measured by enzyme-linked immunosorbent assay(ELISA)2 h later.The expression levels of Rho-associated coiled-coil containing protein kinase I (ROCKI)mRNA of left ventricular myocardium and left gastrocnemius muscle were analyzed by reverse transcriptase polymerase chain reaction(RT-PCR).Results:Compared with the poisoning group,the striated muscle poisoning symptoms were less serious in the pretreated group.Compared with the con-trol group,the level of cTnT in the poisoning group and the pretreated group significantly increased(P <0.05).Compared with the poisoning group,the level of cTnT in the pretreated group significantly decreased(P <0.05).The levels of CK-MM and LDH-M were not obviously different among 3 groups.Compared with the control group,the expression level of ROCKI mRNA of left ventricular myocardium and left gastrocnemius muscle in the poisoning group significantly increased(P <0.05).Com-pared with the poisoning group,the expression level of ROCKI mRNA of left ventricular myocardium and left gastrocnemius muscle in the pretreated group was significantly inhibited(P <0.05).Conclusions:Fasudil can relieve symptoms of AOPP in rats.Fasudil has protective effect on the striated muscle in rats.
