Expression of Indoleamine 2,3-Dioxygenase in the Tissue of Transplanted Kidney under T Cell-Mediated Re-jection
- VernacularTitle:吲哚胺2，3-双加氧酶在T细胞介导的排斥反应移植肾组织中的表达
- Author:
Jia LIU
1
;
Xiaobo WANG
;
Tao GUO
;
Li LIU
;
Guiwen FENG
;
Wenjun SHANG
;
Lei LIU
;
Jinfeng LI
Author Information
1. 河南医学高等专科学校诊断学教研室
- Keywords:
Renal transplantation;
Rejection;
Indoleamine 2,3-dioxygenase;
Biopsy;
Immunohistochemistry
- From:
Chinese Journal of Clinical Medicine
2014;(5):509-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of indoleamine 2,3-dioxygenase (IDO)in the tissue of transplanted kidney un-der T cell-mediated rejection.Methods:A total of 38 cases of tissue specimens of transplanted kidney under T cell-mediated re-jection according to pathological diagnosis from Apr 2008 to Oct 2012 in the First Affiliated Hospital of Zhengzhou University were collected.In addition,10 cases of normal renal biopsy specimens were randomly selected as control group.Mouse anti-hu-man IDO monoclonal antibody with two-step immunohistochemical staining was applied for the detection of IDO expression in the tissue of transplanted kidney under rejection.Results:There was no IDO expression in the normal renal biopsy tissue.Posi-tive expression rate of IDO was 73.7% in the tissue of transplanted kidney under T cell-mediated rejection.There was statisti-cally significant difference(P <0.01).Positive expression rate of IDO in the tissue of transplanted kidney under T cell mediated acute rejection was obviously higher than that under T cell-mediated chronic rejection(P <0.05).The differences in positive ex-pression rate of IDO in transplanted kidney tissues under different pathological grades of T cell-mediated rejection were statisti-cally significant(P <0.05).The expression intensity of IDO was negatively correlated with the grade of T cell-mediated acute rejection(r=-0.696,P <0.05).Conclusions:IDO is involved in the immune regulation after renal transplantation.Higher ex-pression of IDO can significantly reduce T cell-mediated acute rejection.
