Research on the Neative Reulator Role of SPARCi n Glcolsis in Heatocarcinoma Cells
- VernacularTitle:SPARC 在肝癌细胞中负性调控糖酵解作用的研究
- Author:
JIANGFeng
1
;
HUWeiwei
;
Jing LI
;
DINGGang
;
Wei Hon HUA
Author Information
1. 上海交通大学医学院附属新华医院
- Keywords:
HepG2;
Hepatocarcinoma cells;
Secreted protein acidic and rich in cysteine;
Glycolysis
- From:
Chinese Journal of Clinical Medicine
2014;(5):507-508
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between the expression of secreted protein acidic and rich in cysteine (SPARC)and the glycolysis in hepatocarcinoma cells.Methods:HepG2 hepatocarcinoma cells were transfected with SPARC plasmid and SPARC siRNA,respectively.The change of glucose uptake and lactate generation after transfection was assayed by colourimetry method.Results:The glucose uptake and lactate generation decreased in HepG2 transfected with SPARC plas-mid,compared with the control group (P <0.05).However,the glucose uptake and lactate generation increased in HepG2 transfected with siRNA SPARC,compared with the control group(P <0.05).Conclusions:SPARC negatively regulates gly-colysis in hepatocarcinoma cells.
