Effect of Interferon αon the Expression and Activity of Transcription Factor Sp 1 in Hepatocarcinoma Cells
- VernacularTitle:干扰素α对肝癌细胞中转录因子Sp1的表达及活性的影响
- Author:
Qi PAN
1
;
Wanyong CHEN
;
Kai ZHU
;
Jubo ZHANG
;
Yiming ZHAO
;
Xiaodong ZHU
;
Huichuan SUN
;
Lu NG WA
;
Ning REN
Author Information
1. 复旦大学附属中山医院肝外科
- Keywords:
Hepatocarcinoma;
T ranscription factor;
Sp1;
Interferon-α
- From:
Chinese Journal of Clinical Medicine
2014;(3):239-241
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of interferon-αon the expression and activity of transcription factor specificity pro-tein 1(Sp1) in hepatocarcinoma cells .Methods :The effect of interferon-αon the expression and phosphorylation level of Sp1 in hepatocarcinoma cell line M HCC97H were detected by Western blotting .The effect of interferon-α on the activity of Sp1 was analyzed by electrophoretic mobility shift assay (EMSA) .Results :Interferon-α significantly reduced the expression and phos-phorylation level of Sp1 ,and the reducement on phosphorylation was more distinct .Interferon-α also inhibited the activity of Sp1 significantly .Conclusions :Interferon-αinhibits the activity of Sp1 by down regulating the expression and phosphorylation level of Sp1 .Sp1 may be an optional predictor of clinical efficacy of interferon-αon hepatocarcinoma .
