An Improved Model of Mouse Partial Hepatectomy for Study on Liver Regeneration
- VernacularTitle:小鼠肝脏大部分切除后改良再生模型的建立
- Author:
Guoming SHI
1
;
Jiabin CAI
;
Jingxian YU
;
Aiwu KE
;
Zhaoru DONG
;
Chi ZHANG
;
Pengfei ZHANG
;
Shuangjian QIU
;
Huichuan SUN
;
Jian ZHOU
;
Jia FAN
Author Information
1. 复旦大学附属中山医院肝外科
- Keywords:
Mouse model;
Partial hepatectomy;
Liver regeneration
- From:
Chinese Journal of Clinical Medicine
2014;(3):237-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an improved model of mouse partial hepatectomy for study on liver regeneration .Methods :Healthy 8~10 week old male C57BL/6 mice were anesthetized by intraperitoneal injection .Then ,common hepatic pedicle of left lateral lobe of liver and middle lobe of liver was wrapped around with 6-0 proline suture .6-0 proline was fixed on the sur-face of right middle lobe of liver ,which was 0 .2 cm away from the hepatic pedicle .Hepatic pedicle was ligated and left lateral lobe and middle lobe of liver were resected 0 .2 cm away from ligature .The postoperative survival rate and the situation of liver weight vecovery at 3rd day ,7th day ,and 14th day after the operation were observed .Results:An improved mouse model of partial hepatectomy ,of which left lateral lobe of liver and middle lobe of liver were resected ,was established .The weight of removed liver accounted for 66 .7% of total liver and the survival rate of mice was 100% .After liver resection ,the compensa-tory regeneration of remaining liver achieved 58 .2% of the total liver weight at the 3rd day ,78 .0% at the 7th day ,and 93 .2%at the 14th day after the operation .Conclusions :An improved mouse model of partial hepatectomy was successfully established for study on liver regeneration ,which has advantages such as simplicity ,operability and high success rate .
