Non-targeted Metabolomics-based Exploration of Radiation-induced Metabolic Alterations in Mouse Lung Epithelial Cells
10.3779/j.issn.1009-3419.2024.106.28
- VernacularTitle:基于非靶向代谢组学探索辐射诱导的小鼠肺上皮细胞代谢改变
- Author:
FAN HAO
1
,
2
;
GE XIANGWEI
;
ZHOU XIN
;
LI YAO
;
LIU QIAOWEI
;
HU YI
Author Information
1. 100853 北京,中国人民解放军医学院
2. 100071 北京,中国人民解放军总医院第五医学中心肿瘤医学部
- Keywords:
Radiation;
Metabolomics;
MLE12 cells;
Nucleotide metabolism
- From:
Chinese Journal of Lung Cancer
2024;27(10):725-734
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Metabolic change is one of the important characteristics of radiation pneu-monitis.Radiotherapy,as a conventional method for the treatment of thoracic tumors,can not only effectively kill tumor cells,but also cause adverse reactions such as local inflammation and fibrosis,which leads to limited therapeutic effect and profound impact on the quality of life of patients.Therefore,it is of great significance to explore the metabolic changes caused by radio-therapy.The aim of this study was to investigate the effects of X-ray irradiation on the metabolism of a mouse lung epithelial cell line(murine lung epithelial-12,MLE12).Methods MLE12 cells were cultured in vitro and randomly divided into radiation group(IR)and control group(NC).Cells in the IR group were irradiated at a dose of 10 Gy using a Hitachi X-ray irradiator.Cell supernatant samples were collected at 48 h after irradiation.Metabolomic analysis of the samples was performed by liq-uid chromatograph mass spectrometer(LC/MS).Results LC/MS metabolomics analysis revealed the metabolic changes of MLE12 cells at 48 h after irradiation.A total of 38 secretory metabolites were altered in the IR group compared with the NC group.According to the annotation of Kyoto Encyclopedia of Genes and Genomes(KEGG)database,the differential metabo-lites are mainly involved in nucleotide metabolism,amino acid metabolism and lipid metabolism,among which the difference in nucleotide metabolism is the most significant.Conclusion The metabolism ofMLE12 cells was significantly affected by X-ray irradiation,mainly affecting the nucleotide metabolic pathways,including purine and pyrimidine metabolites and related meta-bolic pathways.
