Significance of Epstein-Barr Virus DNA Quantitation in Donors of Hematopoietic Stem Cell Transplantation.
10.3343/kjlm.2010.30.6.554
- Author:
Seungwon JUNG
1
;
Jihyang LIM
;
Byung Sik CHO
;
Hyojin CHAE
;
Myungshin KIM
;
Yonggoo KIM
;
Kyungja HAN
;
Jong Wook LEE
;
Woo Sung MIN
Author Information
1. Department of Laboratory Medicine, The Catholic University of Korea School of Medicine, Seoul, Korea. ljh117@catholic.ac.kr
- Publication Type:Original Article ; English Abstract
- Keywords:
EBV;
Real time PCR;
Hematopoietic stem cell transplantation;
Donor
- MeSH:
Adolescent;
Adult;
Aged;
Child;
DNA, Viral/*blood;
Female;
*Hematopoietic Stem Cell Transplantation;
Herpesvirus 4, Human/*genetics;
Humans;
Male;
Middle Aged;
Polymerase Chain Reaction/*methods;
Tissue Donors;
Transplantation, Homologous;
Viral Load
- From:The Korean Journal of Laboratory Medicine
2010;30(6):554-558
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Epstein-Barr virus (EBV) is a well-known causative agent of various diseases including post-transplant lymphoproliferative disorders. Although the level of EBV viral load in donors is expected to have a direct effect on recipients after hematopoietic stem cell transplantation (HSCT), little has been studied providing a clear evidence for that. We performed EBV DNA quantitation in donors and analyzed the effect of donors' EBV viral load on the recipients after HSCT. METHODS: EBV DNA quantitation of peripheral blood in 94 healthy HSCT donors was performed by real-time PCR. We analyzed the distribution of EBV viral load in HSCT donors and EBV positivity in the recipients transplanted from donors who had detectable EBV. RESULTS: Fifteen HSCT donors (16%) showed positive results in EBV real-time quantitative PCR. EBV viral load was below 500 copies/mL in 5 donors and above 500 (680-11,300) copies/mL in 10 donors. Five of the recipients (33.3%) transplanted from these 15 donors showed positivity in EBV PCR after HSCT. All of the EBV PCR positive recipients were transplanted from donors with viral load of >1,000 copies/mL, and 5 (71%) of 7 donors with viral load of >1,000 copies/mL was associated with posttansplant EBV PCR positivity in the recipients. CONCLUSIONS: Higher levels of EBV viral load in donors appear to be associated with EBV transmission to recipients in HSCT. EBV real-time quantitative PCR may be needed for screening EBV DNA level in HSCT donors.
