Expression and significance of DNMT1 in human keloid fibroblast
10.3760/cma.j.issn.1009-4598.2013.02.010
- VernacularTitle:DNA甲基转移酶1在人瘢痕疙瘩成纤维细胞中的表达及意义
- Author:
E YANG
1
;
Qi-Pa ZOU
;
Heng-Shu ZHANG
Author Information
1. 重庆医科大学附属第一医院整形美容科
- Keywords:
Keloid;
DNA methyltransferase 1;
Fibroblasts
- From:
Chinese Journal of Plastic Surgery
2013;29(2):117-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of DNA methyltransferase 1 (DNMT1) in different parts and formation time of keloid fibroblasts.Methods Specimen were collected from fifty-three patients with keloid.The keloids were grouped according to formation time.Immunohistochemistry was used to assay the expression of DNMT1 in normal skin,invasive part,proliferative part and aged part.RT-PCR was adopted to assay the expression of DNMT1 mRNA in each group.Results DNMT1 is mainly distributed in the nucleus of fibroblasts.The expression of DNMT1protein and DNMT1mRNA in keloid (72％) is higher than that in normal skin fibroblasts (8％) (P =0.001).The expression rate of DNMT1 protein and DNMT1 mRNA in shorter formation time group (100％,Score:10.47 ± 1.85) is higher than a longer formation time group(45％,Score:7.71 + 1.80) (P=0.007).The expression rate of DNMT1 protein and DNMT1 mRNA in the invasive part of keloid (100％,Score:10.47 ± 1.85) is higher than the proliferative part (78％,Score:6.63 ± 1.75) and aged part(38％,Score:5.53 ±1.55) (P=0.001).Conclusions DNMT1 plays an important role in keloid.The expression of DNMT1 tends to decrease along with the increase of the formation time in keloid.It is also related to the part of keloid.DNMT1 plays an important role in the process of keloid growth and is closely related to infiltrative growth in keloid.
