Effects of fentanyl citrate on pain threshold and spinal substance P signaling transduction in a hip fracture model
- VernacularTitle:枸橼酸芬太尼对髋部骨折模型疼痛阈值和脊髓P物质信号传导的影响
- Author:
Xiaoli FENG
1
;
Zhi SUN
;
Junying LIU
;
Lizhen WANG
;
Xinzhe LIU
Author Information
- Keywords: fentanyl citrate; hip fracture; old age; pain; substance P; rats
- From: Journal of Clinical Medicine in Practice 2024;28(21):89-94,99
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effects of fentanyl citrate on pain in aged rats with hip fracture and its underlying mechanisms.Methods Thirty aged rats with hip fracture were divided in-to model group(n=10),fentanyl citrate group(n=10),and fentanyl citrate+Sar-SP group(n=10).Additionally,healthy rats from the same period were included as normal group(n=10).Rats in the fentanyl citrate group received intravenous tail injection of 10 pg/kg fentanyl citrate,while those in the fentanyl citrate+Sar-SP group underwent intrathecal injection of 10 μL Sar-SP for fenta-nyl citrate intervention.Rats in the normal and model groups received intravenous tail injection of an equal volume of saline.Pain threshold,weight-bearing capacity,temperature,and dorsoventral thick-ness of the hind paws were measured before treatment(0h)and at 1,6,24,and 168 h post-treat-ment.Enzyme-linked immunosorbent assay(ELISA)was used to detect levels of tumor necrosis fac-tor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),and glutamic acid(Glu)in the spinal cord tissue of rats.Immunohistochemical staining was employed to observe the positive expression rates of ionized calcium-binding adapter molecule 1(Iba-1),glial fibrillary acidic protein(GFAP),and substance P(SP)in the spinal cord tissue.Real-time fluorescent quantitative polymerase chain re-action(qRT-PCR)was conducted to detect the relative expression levels of SP and tachykinin re-ceptor 1(TACR1)mRNA in the hippocampus tissue of rats.Western blot analysis was performed to assess the relative expression levels of vimentin(VIM),nuclear receptor corepressor 1(NOCR),ciliary neurotrophic factor receptor(CNTFR),and epidermal growth factor receptor(EGFR)pro-teins in the spinal cord tissue.Results Compared with the normal group,rats in the model group exhibited decreased pain threshold and weight-bearing capacity,increased hind paw temperature and dorsoventral thickness,as well as elevated levels of TNF-α,IL-6,IL-1 β,and Glu,increased posi-tive expression rates of Iba-1 and GFAP,heightened positive expression rate and relative mRNA ex-pression levels of SP and TACR1,and augmented relative protein expression levels of VIM,NOCR,CNTFR,and EGFR in the spinal cord tissue at 0 h before treatment and at 1,6,24,and 168 h post-treatment(P＜0.05).Compared with the model group,rats in the fentanyl citrate group showed increased pain threshold and weight-bearing capacity,decreased hind paw temperature and dorsoventral thickness,as well as reduced levels of TNF-α,IL-6,IL-1β,and Glu,decreased posi-tive expression rates of Iba-1 and GFAP,lowered positive expression rate and relative mRNA expres-sion levels of SP and TACR1,and decreased relative protein expression levels of VIM,NOCR,CNTFR,and EGFR in the spinal cord tissue at 1,6,24,and 168 h post-treatment(P＜0.05).Compared with the fentanyl citrate group,rats in the fentanyl citrate+Sar-SP group demonstrated decreased pain threshold and weight-bearing capacity,increased hind paw temperature and dorso-ventral thickness,elevated levels of TNF-α,IL-6,IL-1 β,and Glu,increased positive expression rates of Iba-1 and GFAP,heightened positive expression rate and relative mRNA expression levels of SP and TACR1,and elevated relative protein expression levels of VIM,NOCR,CNTFR,and EGFR in the spinal cord tissue at 1,6,24,and 168 h post-treatment(P＜0.05).Conclusion The im-provement in pain in aged rats with hip fracture by fentanyl citrate may be associated with the reduc-tion of SP expression in the spinal cord that inhibits glial cell activation and exhibits anti-inflammato-ry effects.
