Advancements in Single-cell RNA Sequencing Technology in the Study of the Tumor Microenvironment in Lung Cancer
10.3779/j.issn.1009-3419.2024.101.15
- VernacularTitle:单细胞RNA测序技术在肺癌肿瘤微环境研究中的进展
- Author:
WANG YANHONG
1
,
2
;
LUO BIN
;
WANG ZHUO
;
QUE ZUJUN
;
JIANG LEI
;
TIAN JIANHUI
Author Information
1. 200071 上海,上海中医药大学附属市中医医院肿瘤临床医学中心
2. 200071 上海,上海中医药大学附属市中医医院肿瘤研究所
- Keywords:
Single-cell RNA sequencing;
Lung neoplasms;
Immunotherapy;
Tumor heterogeneity;
Tumor micro-environment
- From:
Chinese Journal of Lung Cancer
2024;27(6):441-450
- CountryChina
- Language:Chinese
-
Abstract:
The immune microenvironment plays a key role in the development and progression of tumors.In recent years,with the rapid advancement of high-throughput sequencing technologies,researchers have gained a deeper under-standing of the composition and function of immune cells in the tumor microenvironment.However,traditional bulk sequenc-ing technologies are limited in resolving heterogeneity at the single-cell level,constraining a comprehensive understanding of the complexity of the tumor microenvironment.The advent of single-cell RNA sequencing technology has brought new opportunities to uncover the heterogeneity of the immune microenvironment in lung cancer.Currently,T-cell-centered im-munotherapy in clinical settings is prone to side effects affecting prognosis,such as immunogenic drug resistance or immune-related pneumonia,with the key factor being changes in the interactions between immune cells and tumor cells in the tumor microenvironment.Single-cell RNA sequencing technology can reveal the origins and functions of different subgroups within the tumor microenvironment from perspectives such as intercellular interactions and pseudotime analysis,thereby discovering new cell subgroups or novel biomarkers,providing new avenues for uncovering resistance to immunotherapy and monitoring therapeutic efficacy.This review comprehensively discusses the newest research techniques and advancements in single-cell RNA sequencing technology for unveiling the heterogeneity of the tumor micro environment after lung cancer immunotherapy,offering insights for enhancing the precision and personalization of immunotherapy.
